Preparation of deuterated arachidonic acid

D. F. Taber; M. A. Phillips; W. C. Hubbard

doi:10.1016/0090-6980(81)90097-6

Preparation of deuterated arachidonic acid

D. F. Taber, M. A. Phillips, W. C. Hubbard

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Arachidonic acid is transformed in vivo to a wide variety of oxygenated metabolites. The limiting factor in the quantitation of these metabolites by GC/MS using selected ion monitoring (SIM) is the availability of suitable stable-isotope labelled internal standards. We report a convenient procedure for the preparation of ²H₈-arachidonic acid, from which the desired deuterated metabolite standards can be produced by a combination of chemical and biological procedures.

Original language	English (US)
Pages (from-to)	349-352
Number of pages	4
Journal	Prostaglandins
Volume	22
Issue number	3
DOIs	https://doi.org/10.1016/0090-6980(81)90097-6
State	Published - Sep 1981
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Endocrinology

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10.1016/0090-6980(81)90097-6

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title = "Preparation of deuterated arachidonic acid",

abstract = "Arachidonic acid is transformed in vivo to a wide variety of oxygenated metabolites. The limiting factor in the quantitation of these metabolites by GC/MS using selected ion monitoring (SIM) is the availability of suitable stable-isotope labelled internal standards. We report a convenient procedure for the preparation of 2H8-arachidonic acid, from which the desired deuterated metabolite standards can be produced by a combination of chemical and biological procedures.",

author = "Taber, {D. F.} and Phillips, {M. A.} and Hubbard, {W. C.}",

note = "Funding Information: This investigation was supported by the National Institutes of Health, GM 15431. We acknowledge support from Biomedical Research Support Grant RR-05424 for MS and NMR instrumentation. We thank Dr. James Hamilton of Hoffman-LaRoche for a generous gift of ETYA,",

year = "1981",

month = sep,

doi = "10.1016/0090-6980(81)90097-6",

language = "English (US)",

volume = "22",

pages = "349--352",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier BV",

number = "3",

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T1 - Preparation of deuterated arachidonic acid

AU - Taber, D. F.

AU - Phillips, M. A.

AU - Hubbard, W. C.

N1 - Funding Information: This investigation was supported by the National Institutes of Health, GM 15431. We acknowledge support from Biomedical Research Support Grant RR-05424 for MS and NMR instrumentation. We thank Dr. James Hamilton of Hoffman-LaRoche for a generous gift of ETYA,

PY - 1981/9

Y1 - 1981/9

N2 - Arachidonic acid is transformed in vivo to a wide variety of oxygenated metabolites. The limiting factor in the quantitation of these metabolites by GC/MS using selected ion monitoring (SIM) is the availability of suitable stable-isotope labelled internal standards. We report a convenient procedure for the preparation of 2H8-arachidonic acid, from which the desired deuterated metabolite standards can be produced by a combination of chemical and biological procedures.

AB - Arachidonic acid is transformed in vivo to a wide variety of oxygenated metabolites. The limiting factor in the quantitation of these metabolites by GC/MS using selected ion monitoring (SIM) is the availability of suitable stable-isotope labelled internal standards. We report a convenient procedure for the preparation of 2H8-arachidonic acid, from which the desired deuterated metabolite standards can be produced by a combination of chemical and biological procedures.

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U2 - 10.1016/0090-6980(81)90097-6

DO - 10.1016/0090-6980(81)90097-6

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AN - SCOPUS:0019810433

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VL - 22

SP - 349

EP - 352

JO - Prostaglandins

JF - Prostaglandins

IS - 3

ER -

Preparation of deuterated arachidonic acid

Abstract

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