Preparation of CDDP incorporated stealth niosomes and their properties after conjugation with BSA

Hong Yang, Ying Cong Zhou, An Ping Deng, Bo Xiao, Jing Jing Huang, Ruo Bing Chao

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To prepare stealth niosomes and BSA-conjugated stealth niosome of CDDP, and to compare their physicochemical and in vitro biological properties. Methods: Pluronic F68 and BSA were coupled in the presence of NHS. Then niosomes were prepared using modified injection-extrude method. The particle diameters were evaluated under microscope. The entrapment efficiency and stability of niosomes were determined by dialysis method and UV spectrophotometry. The toxicity and uptake to MPM cells were examined. Results: The particle diameters of the two kinds of niosomes were 3.48 and 4.49 μm, and the entrapment efficiency were 51.54% and 44.02%, respectively. After one week, their leaking percentage was 20.97% and 24.09%, respectively. Their cytotoxicities were the same to the raw drug. Due to conjugation with BSA, the uptake percentage of MPM cells increased. Conclusion: This preparative technology was simple and mild, which potentially provides a sound base for the coupling of mAb to form immune stealth niosomes.

Original languageEnglish (US)
Pages (from-to)668-671
Number of pages4
JournalChinese Journal of New Drugs
Volume18
Issue number7
StatePublished - Apr 15 2009

Keywords

  • Cis-diamminedichloroplatinum (CDDP)
  • Immune niosomes
  • Niosomes
  • Stealth niosome

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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