Preparation and in vitro analysis of microcapsule thalidomide formulation for targeted suppression of TNF-α

Terrence Metz, Tasima Haque, Hongmei Chen, Satya Prakash, Devendra Amre, Sujata Das

Research output: Contribution to journalArticle

Abstract

Recent studies have implicated the cytokine tumor necrosis factor-alpha (TNF-α) in the inflammation associated with Crohn's disease (CD). Thalidomide has been shown to decrease this inflammation by the suppression of TNF-α secretion. However, side effects associated with thalidomide have precluded its widespread usage. In the present study we investigated the efficacy of a "targeted delivery approach" for thalidomide at the site of inflammation. We observed that alginate-poly-l-lysine-alginate (APA) polymer-based microcapsule formulations that encapsulate thalidomide could be designed. These capsules could be delivered at target sites where they almost entirely suppress TNF-α secretion in lipopolysaccharide activated RAW 264.7 macrophage cells in vitro. These findings indicate that targeted delivery of thalidomide using APA capsules could facilitate its usage in reducing the inflammation associated with chronic conditions such as Crohn's disease and ulcerative colitis.

Original languageEnglish (US)
Pages (from-to)331-337
Number of pages7
JournalDrug Delivery
Volume13
Issue number5
DOIs
StatePublished - Oct 1 2006

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Keywords

  • Alginate
  • Artificial Cells
  • Crohn's Disease
  • Inflammatory Bowel Disease
  • Microcapsule
  • Polylysine
  • TNF-α
  • Thalidomide

ASJC Scopus subject areas

  • Pharmaceutical Science

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