Preparation and identification of per a 5 as a novel American cockroach allergen

Ji Fu Wei, Haiwei Yang, Dongning Li, Peisong Gao, Shaoheng He

Research output: Contribution to journalArticle

Abstract

Glutathione S-transferase (GST) from various arthropods can elicit allergic reactions. In the present study, Per a 5, a GST, was cloned from American cockroach (CR) and expressed in both baculovirus-infected insect cell (iPer a 5) and E. coli expression (bPer a 5) systems. The secondary structures were predicted to be 45.93 and 8.69% of α-helix β-sheets in iPer a 5 and 42.54 and 8.49% of α-helix and β-sheets in bPer a 5, respectively. It is found that 4 out of 16 (25%) sera from American CR allergy patients reacted to both bPer a 9 and iPer a 9 as assessed by ELISA and Western blotting analysis, confirming that Per a 5 is not a major allergen of American CR. Induction of upregulated expression of CD63 and CCR3 on passively sensitized human basophils (sera from American CR allergy patients) by approximately up to 4.5- and 3.2-fold indicates that iPer a 5 and bPer a 5 are functionally active. Recombinant Per a 5 (rPer a 5) should be a useful tool for studying and understanding the role of Per a 5 in CR allergy.

Original languageEnglish (US)
Article number591468
JournalMediators of Inflammation
Volume2014
DOIs
StatePublished - 2014

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Periplaneta
Allergens
Hypersensitivity
Glutathione Transferase
Cockroaches
Basophils
Arthropods
Baculoviridae
Serum
Insects
Western Blotting
Enzyme-Linked Immunosorbent Assay
Escherichia coli

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Medicine(all)

Cite this

Preparation and identification of per a 5 as a novel American cockroach allergen. / Wei, Ji Fu; Yang, Haiwei; Li, Dongning; Gao, Peisong; He, Shaoheng.

In: Mediators of Inflammation, Vol. 2014, 591468, 2014.

Research output: Contribution to journalArticle

Wei, Ji Fu ; Yang, Haiwei ; Li, Dongning ; Gao, Peisong ; He, Shaoheng. / Preparation and identification of per a 5 as a novel American cockroach allergen. In: Mediators of Inflammation. 2014 ; Vol. 2014.
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abstract = "Glutathione S-transferase (GST) from various arthropods can elicit allergic reactions. In the present study, Per a 5, a GST, was cloned from American cockroach (CR) and expressed in both baculovirus-infected insect cell (iPer a 5) and E. coli expression (bPer a 5) systems. The secondary structures were predicted to be 45.93 and 8.69{\%} of α-helix β-sheets in iPer a 5 and 42.54 and 8.49{\%} of α-helix and β-sheets in bPer a 5, respectively. It is found that 4 out of 16 (25{\%}) sera from American CR allergy patients reacted to both bPer a 9 and iPer a 9 as assessed by ELISA and Western blotting analysis, confirming that Per a 5 is not a major allergen of American CR. Induction of upregulated expression of CD63 and CCR3 on passively sensitized human basophils (sera from American CR allergy patients) by approximately up to 4.5- and 3.2-fold indicates that iPer a 5 and bPer a 5 are functionally active. Recombinant Per a 5 (rPer a 5) should be a useful tool for studying and understanding the role of Per a 5 in CR allergy.",
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