Preparation and Evaluation of ZD2 Peptide 64 Cu-DOTA Conjugate as a Positron Emission Tomography Probe for Detection and Characterization of Prostate Cancer

Zheng Han, Olga Sergeeva, Sarah Roelle, Han Cheng, Songqi Gao, Yajuan Li, Zhenghong Lee, Zheng Rong Lu

Research output: Contribution to journalArticle

Abstract

Positron emission tomography (PET) is a sensitive modality for cancer molecular imaging. We aim to develop a PET probe for sensitive detection and risk stratification of prostate cancer by targeting an abundant microenvironment oncoprotein, extradomain-B fibronectin (EDB-FN). The probe consists of a small ZD2 peptide specific to EDB-FN and a 64 Cu-DOTA chelate. The probe was synthesized using standard solid-phase peptide chemistry and chelated to 64 Cu prior to imaging. PET images were acquired at 4 and 22 h after intravenously injecting a 200 μCi probe into mice bearing human PC3 and LNCaP tumors, which represent highly aggressive and slow-growing prostate tumors, respectively. At 4 and 22 h postinjection, tumors could be clearly identified in the PET images. A significant higher signal was observed in PC3 tumors than in LNCaP tumors at 22 h (p = 0.01). Probe accumulation was also higher in PC3 tumors at 24 h. These data demonstrated that PET molecular imaging of EDB-FN in the tumor microenvironment of prostate cancer allows efficient differentiation of PC3 and LNCaP tumors in vivo. The ZD2 peptide-targeted PET probe shows potential in the detection and characterization of high-risk prostate cancer to improve the clinical management of prostate cancer.

Original languageEnglish (US)
Pages (from-to)1185-1190
Number of pages6
JournalACS Omega
Volume4
Issue number1
DOIs
StatePublished - Jan 14 2019

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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