This chapter discusses techniques for the preparation and characterization of mitochondrial and submitochondrial fractions from liver and liver-derived tissues. The chapter represents the most detailed account of preparative procedures available for mitochondria and submitochondrial particles of rat liver. The chapter initially summarizes several methods for isolating rat liver mitochondria. Some of these methods yield type-1 mitochondria (partially purified but intact), and some yield type-2 mitochondria (highly purified). The two most common tests used to determine the quality of mitochondrial and submitochondrial preparations involve an examination of their respiratory characteristics in a small chamber equipped with an oxygen electrode, and an examination of their structural and morphological features under the electron microscope. The chapter presents flow diagrams that include many of the methods to obtain an overall view of a given procedure. The methodology involved in preparing mitochondria for evaluation in the electron microscope is summarized here, together with a few statements about what each step involves and why it is carried out.
ASJC Scopus subject areas
- Cell Biology