5-, 8-, 9-, 11-, 12- and 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H2O2 and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (μ Porasil) column or on a reversed phase (μ Bondapak C18) column. The yield of 5-HPETE was considerably greater when the μ Bondapak C18 column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20°C, the HPETEs were stable for several months.
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