Preparation and analysis of uniquely positioned mononucleosomes.

Daria A. Gaykalova, Olga I. Kulaeva, Vladimir A. Bondarenko, Vasily M. Studitsky

Research output: Contribution to journalArticlepeer-review

Abstract

Short DNA fragments containing single, uniquely positioned nucleosome cores have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, transcription, DNA repair and ATP-dependent chromatin remodeling. In many cases such simple model templates faithfully recapitulate numerous important aspects of these processes. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes that are uniquely positioned on 150-600 bp DNA fragments.

Original languageEnglish (US)
Pages (from-to)109-123
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume523
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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