Prenatal diagnosis of hemoglobinopathies: A potential application of high performance liquid chromatography

Blanche P. Alter, Decherd D. Stomp

Research output: Contribution to journalArticlepeer-review

Abstract

High performance liquid chromatography was compared with carboxymethyl cellulose column chromatography data for analysis of globin chain composition and biosynthetic ratios in fetal blood. A formula was derived for mathematical correction for losses of small amounts of globin protein on the high performance liquid chromatography column. Radioactive globin chain ratios by high performance liquid chromatography are equivalent to those by carboxymethyl cellulose column chromatography, but only when carrier Hb A is added. We conclude that high performance liquid chromatography provides a suitable alternative to carboxymethyl cellulose columns, and has several advantages.

Original languageEnglish (US)
Pages (from-to)341-352
Number of pages12
JournalHemoglobin
Volume11
Issue number4
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Hematology
  • Genetics(clinical)
  • Clinical Biochemistry
  • Biochemistry, medical
  • Biochemistry

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