Preliminary x‐ray diffraction analysis of HhaII endonuclease‐DNA cocrystals

Srinivasan Chandrasegaran, Hamilton O. Smith, Mario L. Amzel, Xavier Ysern

Research output: Contribution to journalArticle

Abstract

HhaII restriction endonuclease purified from an overproducing recombinant E. coli clone has been cocrystallized with a heptanucleotide duplex, d‐GGAGTCC:GGACTCC. The cocrystals are monoclonic and belong to the space group C2. The unit cell dimensions are a = 199.0±1.0 Å, b = 100.0±0.5 Å, c = 80.3±0.4 Å, and β = 101.0±1.0°. There appear to be two dimers per asymmetric unit and the crystals diffract to 4‐Å resolution.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalProteins: Structure, Function, and Bioinformatics
Volume1
Issue number3
DOIs
StatePublished - Mar 1986

Keywords

  • overproducer clone
  • protein‐DNA interaction
  • sequence‐specific recognition

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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