TY - JOUR
T1 - Preliminary x‐ray diffraction analysis of HhaII endonuclease‐DNA cocrystals
AU - Chandrasegaran, Srinivasan
AU - Smith, Hamilton O.
AU - Amzel, Mario L.
AU - Ysern, Xavier
PY - 1986/3
Y1 - 1986/3
N2 - HhaII restriction endonuclease purified from an overproducing recombinant E. coli clone has been cocrystallized with a heptanucleotide duplex, d‐GGAGTCC:GGACTCC. The cocrystals are monoclonic and belong to the space group C2. The unit cell dimensions are a = 199.0±1.0 Å, b = 100.0±0.5 Å, c = 80.3±0.4 Å, and β = 101.0±1.0°. There appear to be two dimers per asymmetric unit and the crystals diffract to 4‐Å resolution.
AB - HhaII restriction endonuclease purified from an overproducing recombinant E. coli clone has been cocrystallized with a heptanucleotide duplex, d‐GGAGTCC:GGACTCC. The cocrystals are monoclonic and belong to the space group C2. The unit cell dimensions are a = 199.0±1.0 Å, b = 100.0±0.5 Å, c = 80.3±0.4 Å, and β = 101.0±1.0°. There appear to be two dimers per asymmetric unit and the crystals diffract to 4‐Å resolution.
KW - overproducer clone
KW - protein‐DNA interaction
KW - sequence‐specific recognition
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U2 - 10.1002/prot.340010309
DO - 10.1002/prot.340010309
M3 - Article
C2 - 2835764
AN - SCOPUS:0022804572
SN - 0887-3585
VL - 1
SP - 263
EP - 266
JO - Proteins: Structure, Function, and Bioinformatics
JF - Proteins: Structure, Function, and Bioinformatics
IS - 3
ER -