Preliminary Study on Diffuse Axonal Injury by Fourier Transform Infrared Spectroscopy Histopathology Imaging

Tiantong Yang, Guanglong He, Xiang Zhang, Lin Chang, Haidong Zhang, Mary G. Ripple, David R. Fowler, Ling Li

Research output: Contribution to journalArticlepeer-review

Abstract

The objective of this study was to evaluate the application of Fourier transform infrared (FTIR) spectroscopy for detecting diffuse axonal injury (DAI) in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and β-amyloid precursor protein (β-APP) immunohistochemistry stains and were also studied with FTIR. The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and β-APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques.

Original languageEnglish (US)
Pages (from-to)231-235
Number of pages5
JournalJournal of Forensic Sciences
Volume59
Issue number1
DOIs
StatePublished - Jan 2014
Externally publishedYes

Keywords

  • Animal study
  • Diffuse axonal injury
  • Forensic pathology
  • Forensic science
  • Fourier transform infrared spectroscopy
  • Traumatic brain injury

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics

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