In the course of studies on the incorporation of sodium [35S]sulfate into sulfatide in patients with neurological disorders, a metabolically active substance was found which resembles sulfatide both in solubility and behavior on column chromatography but is separable from it on thin-layer chromatography, A method was devised for the isolation of larger quantities of this material. It was found to have the same melting point and Chromatographie properties as authentic cholesterol sulfate and strongly resembles it in its infrared spectrum, Mild acid hydrolysis yielded a substance identical to cholesterol in its melting point, and infrared and nuclear magnetic spectra. A survey of human tissues revealed the presence of small amounts of cholesterol sulfate in plasma, bile, urine, liver, kidney, and larger quantities in feces. It was also found in the brain of a 4-day-old infant. Intravenously administered sodium [35S]sulfate was rapidly incorporated into cholesterol sulfate. Until recently, cholesterol sulfate had not been isolated from any biological material. This study demonstrates that it is metabolically active and that it is present in a variety of human tissues and body fluids.
|Original language||English (US)|
|Number of pages||10|
|Journal||Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism|
|State||Published - Feb 1 1966|
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