Preliminary observations on skeletal muscle adaptation and plasticity in homer 2-/- mice

Paola Lorenzon, Sandra Furlan, Barbara Ravara, Alessandra Bosutti, Gabriele Massaria, Annalisa Bernareggi, Marina Sciancalepore, Gabor Trautmann, Katharina Block, Dieter Blottner, Paul F. Worley, Sandra Zampieri, Michele Salanova, Pompeo Volpe

Research output: Contribution to journalArticlepeer-review

Abstract

Homer represents a diversified family of scaffold and transduction proteins made up of several isoforms. Here, we present preliminary observations on skeletal muscle adaptation and plasticity in a transgenic model of Homer 2-/- mouse using a multifaceted approach entailing morphometry, quantitative RT-PCR, confocal immunofluorescence, and electrophysiology. Morphometry shows that Soleus muscle (SOL), at variance with Extensor digitorum longus muscle (EDL) and Flexor digitorum brevis muscle (FDB), displays sizable reduction of fibre cross-sectional area compared to the WT counterparts. In SOL of Homer 2-/- mice, quantitative RT-PCR indicated the upregulation of Atrogin-1 and Muscle ring finger protein 1 (MuRF1) genes, and confocal immunofluores-cence showed the decrease of neuromuscular junction (NMJ) Homer content. Electrophysiological measurements of isolated FDB fibres from Homer 2-/- mice detected the exclusive presence of the adult ε-nAChR isoform excluding denervation. As for NMJ morphology, data were not conclusive, and further work is needed to ascertain whether the null Homer 2 phenotype induces any endplate remodelling. Within the context of adaptation and plasticity, the present data show that Homer 2 is a co-regulator of the normotrophic status in a muscle specific fashion.

Original languageEnglish (US)
Article number642
JournalMetabolites
Volume11
Issue number9
DOIs
StatePublished - Sep 2021

Keywords

  • Atrophy
  • Homer 2
  • Neuromuscular junction
  • Skeletal muscle

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

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