Preliminary characterization of Yor180Cp: Identification of a novel peroxisomal protein of Saccharomyces cerevisiae involved in fatty acid metabolism

Brian V. Geisbrecht, Kerstin Schulz, Katja Nau, Michael T. Geraghty, Horst Schulz, Ralf Erdmann, Stephen J. Gould

Research output: Contribution to journalArticlepeer-review

Abstract

Here we report the preliminary characterization of Yor180Cp, a novel peroxisomal protein involved in fatty acid metabolism in the yeast Saccharomyces cerevisiae. A computer-based screen identified Yor180Cp as a putative peroxisomal protein, and Yor180Cp targeted GFP to peroxisomes in a PEX8-dependent manner. Yor180Cp was also detected by mass spectrometric analysis of an HPLC-separated extract of yeast peroxisomal matrix proteins. YOR180C is upregulated during growth on oleic acid, and deletion of YOR180C from the yeast genome resulted in a mild but significant growth defect on oleic acid, indicating a role for Yor180Cp in fatty acid metabolism. In addition, we observed that yorl80cΔ cells fail to efficiently import the enzyme Δ32-enoyl-CoA isomerase (Eci1p) to peroxisomes. This result suggested that Yor180Cp might associate with Eci1p in vivo, and a Yor180Cp-Eci1p interaction was detected using the yeast two-hybrid system. Potential roles for Yor180Cp in peroxisomal fatty acid metabolism are discussed.

Original languageEnglish (US)
Pages (from-to)28-34
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume260
Issue number1
DOIs
StatePublished - Jun 24 1999

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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