TY - JOUR
T1 - Preliminary characterization of Yor180Cp
T2 - Identification of a novel peroxisomal protein of Saccharomyces cerevisiae involved in fatty acid metabolism
AU - Geisbrecht, Brian V.
AU - Schulz, Kerstin
AU - Nau, Katja
AU - Geraghty, Michael T.
AU - Schulz, Horst
AU - Erdmann, Ralf
AU - Gould, Stephen J.
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health DK45787 and HD10981 to S.J.G., by Deutsche Forschungs-gemeinschaft Grant ERA78/2-1 to R.E., by United States Public Health Service Grants HL30847 from the NHLBI, National Institutes of Health to H.S., and RR03060 to Research Centers of Minority Institutions. We thank James C. Morrell for expert technical assistance.
PY - 1999/6/24
Y1 - 1999/6/24
N2 - Here we report the preliminary characterization of Yor180Cp, a novel peroxisomal protein involved in fatty acid metabolism in the yeast Saccharomyces cerevisiae. A computer-based screen identified Yor180Cp as a putative peroxisomal protein, and Yor180Cp targeted GFP to peroxisomes in a PEX8-dependent manner. Yor180Cp was also detected by mass spectrometric analysis of an HPLC-separated extract of yeast peroxisomal matrix proteins. YOR180C is upregulated during growth on oleic acid, and deletion of YOR180C from the yeast genome resulted in a mild but significant growth defect on oleic acid, indicating a role for Yor180Cp in fatty acid metabolism. In addition, we observed that yorl80cΔ cells fail to efficiently import the enzyme Δ3,Δ2-enoyl-CoA isomerase (Eci1p) to peroxisomes. This result suggested that Yor180Cp might associate with Eci1p in vivo, and a Yor180Cp-Eci1p interaction was detected using the yeast two-hybrid system. Potential roles for Yor180Cp in peroxisomal fatty acid metabolism are discussed.
AB - Here we report the preliminary characterization of Yor180Cp, a novel peroxisomal protein involved in fatty acid metabolism in the yeast Saccharomyces cerevisiae. A computer-based screen identified Yor180Cp as a putative peroxisomal protein, and Yor180Cp targeted GFP to peroxisomes in a PEX8-dependent manner. Yor180Cp was also detected by mass spectrometric analysis of an HPLC-separated extract of yeast peroxisomal matrix proteins. YOR180C is upregulated during growth on oleic acid, and deletion of YOR180C from the yeast genome resulted in a mild but significant growth defect on oleic acid, indicating a role for Yor180Cp in fatty acid metabolism. In addition, we observed that yorl80cΔ cells fail to efficiently import the enzyme Δ3,Δ2-enoyl-CoA isomerase (Eci1p) to peroxisomes. This result suggested that Yor180Cp might associate with Eci1p in vivo, and a Yor180Cp-Eci1p interaction was detected using the yeast two-hybrid system. Potential roles for Yor180Cp in peroxisomal fatty acid metabolism are discussed.
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U2 - 10.1006/bbrc.1999.0860
DO - 10.1006/bbrc.1999.0860
M3 - Article
C2 - 10381339
AN - SCOPUS:0033600137
SN - 0006-291X
VL - 260
SP - 28
EP - 34
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -