Preliminary characterization of enterooxyntic activity on the guinea pig oxyntic cell

W. E. Strodel, W. A. Walker, B. S. Vinik, A. Heldsinger, Frederic Eckhauser

Research output: Contribution to journalArticle

Abstract

Stimuli originating in the small intestine enhance gastric acid secretion, an effect termed the intestinal phase of gastric secretion. A humoral agent, enterooxyntin (EO) may mediate this effect. Mechanical distension of an ex vivo-perfused segment of canine jejunum caused the release of EO measured by cytochemical quantification of hydroxyl ion production (HIP) in guinea pig gastric oxyntic cells, an index of acid secretion. The H2 receptor antagonist, cimetidine (10-5M), and diamine oxidase, which hydrolyzes endogenous histamine, reduced HIP by 60% and 48%, respectively. Atropine (10-5M), the muscarinic cholinergic antagonist, reduced HIP by 75%. EO-induced HIP was also inhibited partially by the Ca2+ antagonist EGTA (10-6 M) and by blockade of calcium channels with LaCl3 (10-6 M). EO does not appear to operate through any single pathway. EO may be a single substance, different from gastrin, or a mixture of substances that have simulatory effects on the oxyntic cell. Its action on the oxyntic cell is apparently mediated by both histaminergic and cholinergic pathways. Since neither cimetidine nor atrophine completely inhibited EO-induced HIP, a direct effect of EO on the oxyntic cell seems likely and appears to depend on Ca2+. Isolation and purification of EO will be necessary to better assess the potency, efficacy, and the detailed cellular mechanisms of EO action.

Original languageEnglish (US)
Pages (from-to)376-381
Number of pages6
JournalEndocrinology
Volume117
Issue number1
StatePublished - 1985
Externally publishedYes

Fingerprint

Gastric Parietal Cells
Guinea Pigs
Cimetidine
Stomach
Amine Oxidase (Copper-Containing)
Histamine H2 Receptors
Muscarinic Antagonists
Egtazic Acid
Gastric Acid
Gastrins
Jejunum
Calcium Channels
Atropine
Cholinergic Agents
Histamine
Small Intestine
Canidae
hydroxide ion
Acids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Strodel, W. E., Walker, W. A., Vinik, B. S., Heldsinger, A., & Eckhauser, F. (1985). Preliminary characterization of enterooxyntic activity on the guinea pig oxyntic cell. Endocrinology, 117(1), 376-381.

Preliminary characterization of enterooxyntic activity on the guinea pig oxyntic cell. / Strodel, W. E.; Walker, W. A.; Vinik, B. S.; Heldsinger, A.; Eckhauser, Frederic.

In: Endocrinology, Vol. 117, No. 1, 1985, p. 376-381.

Research output: Contribution to journalArticle

Strodel, WE, Walker, WA, Vinik, BS, Heldsinger, A & Eckhauser, F 1985, 'Preliminary characterization of enterooxyntic activity on the guinea pig oxyntic cell', Endocrinology, vol. 117, no. 1, pp. 376-381.
Strodel, W. E. ; Walker, W. A. ; Vinik, B. S. ; Heldsinger, A. ; Eckhauser, Frederic. / Preliminary characterization of enterooxyntic activity on the guinea pig oxyntic cell. In: Endocrinology. 1985 ; Vol. 117, No. 1. pp. 376-381.
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