Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes

Susan E. Lanzendorf, Jacob F. Mayer, James Toner, Sergio Oehninger, David S. Saffan, Suheil Muasher

Research output: Contribution to journalArticlepeer-review

70 Scopus citations


Objective: To determine if frozen-thawed donor oocytes could be used to provide cytoplasm for transfer into patients' oocytes to improve subsequent embryonic development. Design: Prospective evaluation of the procedure in consenting IVF patients. Setting: Assisted reproductive technology program. Patient(s): The study was open to consenting IVF patients (of any age) with a history of poor embryo quality or those couples in which the wife's age was ≥40 years. Intervention(s): Transfer of donor egg cytoplasm from frozen- thawed oocytes into the oocytes of infertile recipients. Main Outcome Measure(s): Donor oocyte survival following cryopreservation, fertilization following cytoplasmic transfer into recipient oocytes, embryo quality, and pregnancy outcome. Result(s): Oocytes collected from four donors were cryopreserved and 61% (28/46) survived the thaw procedure. Cytoplasmic transfer was performed on the eggs of four patients, with fertilization occurring in 70.3% (26/37). Twin pregnancy was established in one patient (35 years of age) with a history of poor embryo quality. Conclusion(s): Cryopreserved donor oocytes may provide a source of cytoplasm for transfer into recipient oocytes, eliminating the need for cycle synchronization between donor and infertile patient.

Original languageEnglish (US)
Pages (from-to)575-577
Number of pages3
JournalFertility and sterility
Issue number3
StatePublished - Mar 1999
Externally publishedYes


  • Cytoplasmic transfer
  • In vitro fertilization
  • Intracytoplasmic sperm injection

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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