Preferential suppression of insulin‐stimulated proliferation of cultured hepatocytes by somatostatin: Evidence for receptor‐mediated growth regulation

Piyush C. Kothary, Norihiro Kokudo, F. E. Eckhauser, John Delvalle, Steven E. Raper

Research output: Contribution to journalArticlepeer-review

Abstract

The role of somatostatin (SS‐14) in the regulation of rat liver regeneration was examined by using thymidine incorporation into hepatocyte DNA labeled with tritiated thymidine, a nuclear‐labeling index, and the binding of 125I‐tyr11‐SS‐14 to hepatocytes isolated at various times after partial hepatectomy. The data demonstrated no suppressive effect of SS‐14 on insulin and glucagon‐stimulated thymidine incorporation into hepatocyte DNA as early as 2 h after partial hepatectomy. These data were substantiated by a nuclear labeling index studies. At 2 h, 125I‐tyr11‐SS‐14 binding to its specific sites on isolated hepatocytes was undetectable. There was a time‐dependent increase in binding of 125I‐tyr11‐SS‐14 to hepatocytes obtained at various times after partial hepatectomy. There was a significant decrease in the number of binding sites after partial hepatectomy as determined by Scatchard analysis. The data were supported by autoradiography analysis of affinity labeled 125I‐tyr11‐SS‐14‐binding protein complex followed by SDS‐PAGE. SS‐14 also inhibited intracellular cAMP in hepatocytes obtained at 18 h after hepatectomy. The data are consistent with the hypothesis that SS‐14 participates via its own receptor in the regulation of the liver regeneration. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)258-265
Number of pages8
JournalJournal of cellular biochemistry
Volume59
Issue number2
DOIs
StatePublished - Oct 1995
Externally publishedYes

Keywords

  • cell growth
  • liver regeneration
  • receptor
  • somatostatin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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