Preferential recruitment of activated, memory T lymphocytes into skin chamber fluids during human cutaneous late-phase allergic reactions

Sabine Werfel, William Massey, Lawrence M. Lichtenstein, Bruce S. Bochner

Research output: Contribution to journalArticle

Abstract

To determine whether activated memory T lymphocytes (CD3+ CD4+ CD45RO+ CD25+ HLA-DR+) preferentially accumulate during the cutaneous IgE-dependent late-phase response, we challenged 10 atopic patients with allergen and monitored the cellular influx for 12 hours with a skin blister chamber model. Four patients with allergy were also challenged with irrelevant allergen in control experiments. Histamine release and the size of the cutaneous late-phase response were measured. Light microscopic analysis and phenotyping of recruited and blood lymphocytes were performed with immunofluorescence and flow cytometry. Antigen challenge induced significant histamine release, a macroscopic late-phase response, and significant cellular influx in the appropriately challenged patients with allergy but not in control subjects. In the control group, only limited phenotypic analyses could be performed, which demonstrated an equivalent percentage of CD3+ CD4+ cells in the chamber fluids compared with blood. In contrast, a higher proportion of CD4+ T lymphocytes and a lower proportion of CD8+ T lymphocytes accumulated in chamber fluids during the late-phase response compared with that present in blood. The vast majority of the recruited T lymphocytes expressed a memory phenotype (CD45RO+) with enhanced percentages of CD25+ and HLA-DR+ cells. Also, these cells had increased levels of very late antigen-4 (CD49d/CD29) and reduced levels of L-selectin compared with the same cells in blood. These results demonstrate that allergen challenge of the skin in patients with allergy leads to the preferential accumulation of activated, memory T lymphocytes. The mechanism by which these cells are selectively recruited during cutaneous allergic inflammation remains to be determined. (J Allergy Clin Immunol 1995;96:57-65.).

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalThe Journal of Allergy and Clinical Immunology
Volume96
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Hypersensitivity
T-Lymphocytes
Skin
Allergens
Integrin alpha4beta1
Histamine Release
HLA-DR Antigens
L-Selectin
Blister
Immunoglobulin E
Fluorescent Antibody Technique
Blood Cells
Flow Cytometry
Lymphocytes
Inflammation
Phenotype
Light
Antigens
Control Groups

Keywords

  • CD25
  • CD45RO
  • cutaneous allergic inflammation
  • HLA-DR
  • late-phase response
  • Lymphocyte homing
  • memory T lymphocyte
  • skin chamber

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Preferential recruitment of activated, memory T lymphocytes into skin chamber fluids during human cutaneous late-phase allergic reactions. / Werfel, Sabine; Massey, William; Lichtenstein, Lawrence M.; Bochner, Bruce S.

In: The Journal of Allergy and Clinical Immunology, Vol. 96, No. 1, 1995, p. 57-65.

Research output: Contribution to journalArticle

Werfel, Sabine ; Massey, William ; Lichtenstein, Lawrence M. ; Bochner, Bruce S. / Preferential recruitment of activated, memory T lymphocytes into skin chamber fluids during human cutaneous late-phase allergic reactions. In: The Journal of Allergy and Clinical Immunology. 1995 ; Vol. 96, No. 1. pp. 57-65.
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