Precursor CTL Specific for Retrovirus-Induced T Lymphoma Are Found at High Frequency in Unprimed Syngeneic Mice

Michel P. Moutschen, Hua Shan, Bruce J. Giantonio, Michael A. Bookman

Research output: Contribution to journalArticlepeer-review

Abstract

The retrovirus-induced RBL5 lymphoma can be rejected by adoptive transfer of noncytolytic CD4+ Th1 lymphocytes in normal hosts, without a requirement for transfer of specific CD8+ CTL. Therefore, we hypothesized that host precursor CTL (pCTL) might cooperate with transferred CD4+ Th1 cells to mediate tumor rejection. To evaluate this hypothesis, lymphocytes from non-immunized mice were analyzed for cytolytic activity after short-term bulk lymphocyte tumor culture (BLTC) with rIL-2 (5 U/ml). BLTC induced the differentiation of anti-RBL5 CTL distinct from non-MHC-restricted LAK. These effectors were CD8+, TCR α/β+, and utilized the CD3-TCR complex for MHC class I-restricted lysis. The majority of pCTL were found within the CD44/PgP-1hi population of memory/activated lymphocytes. However, there was no serologic evidence for prior exposure to RBLS-related tumor or viral Ags. CTL activity was susceptible to partial blockade with mAbs directed against CD8 and MHC class I, suggesting a relatively low-affinity Ag-TCR interaction. These data are most consistent with the recruitment of a population of Ag-specific, but cross-reactive, pCTL during BLTC.

Original languageEnglish (US)
Pages (from-to)367-377
Number of pages11
JournalCellular Immunology
Volume153
Issue number2
DOIs
StatePublished - Feb 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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