Preconditioning of the rat random-pattern skin flap: Modulation by opioids

S. Kiumehr, Shadpour Demehri, S. Rabbani, S. Amanpour, M. A. Mohagheghi, A. R. Dehpour

Research output: Contribution to journalArticle

Abstract

Opioid receptors have been implicated in protecting several organ systems from ischaemic events. The authors have studied the effects of opioid receptors on random-pattern skin flap survival. Sixty-nine male Sprague-Dawley rats were used. Bipedicled dorsal skin flaps (2×8 cm) were elevated at the midline. Different doses of morphine (0.01, 0.1, 1 and 5 mg/flap) were administered locally in the cranial half of the flap and systemically through intraperitoneal injections (5 and 10 mg/kg). In another experiment, 0.4 mg/flap of naloxone was injected followed by 5 mg/flap injection of morphine to determine whether the effect of morphine is receptor mediated. The role of the opioid receptors in the ischaemic preconditioning (IPC) phenomenon was investigated by administration of naloxone (0.4 mg/flap) 1 h before clamping the cranial pedicle for 20 min followed by 40 min of reperfusion. Appropriate control groups were included. The cranial pedicle was cut 2 h after saline or drug administration in all groups and flap survival area was evaluated on the seventh postoperative day. Local administration of morphine in higher doses (1 and 5 mg/flap) significantly reduced the amount of flap necrosis when compared to that of the control cohort (P

Original languageEnglish (US)
Pages (from-to)58-64
Number of pages7
JournalBritish Journal of Plastic Surgery
Volume58
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

Opioid Receptors
Morphine
Opioid Analgesics
Naloxone
Skin
Ischemic Preconditioning
mu Opioid Receptor
Intraperitoneal Injections
Constriction
Reperfusion
Sprague Dawley Rats
Necrosis
Control Groups
Injections
Pharmaceutical Preparations

Keywords

  • Morphine
  • Opioid receptors
  • Preconditioning
  • Random flap
  • Rat
  • Skin

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

Preconditioning of the rat random-pattern skin flap : Modulation by opioids. / Kiumehr, S.; Demehri, Shadpour; Rabbani, S.; Amanpour, S.; Mohagheghi, M. A.; Dehpour, A. R.

In: British Journal of Plastic Surgery, Vol. 58, No. 1, 01.2005, p. 58-64.

Research output: Contribution to journalArticle

Kiumehr, S. ; Demehri, Shadpour ; Rabbani, S. ; Amanpour, S. ; Mohagheghi, M. A. ; Dehpour, A. R. / Preconditioning of the rat random-pattern skin flap : Modulation by opioids. In: British Journal of Plastic Surgery. 2005 ; Vol. 58, No. 1. pp. 58-64.
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