Preconditioning of human smooth muscle cells via cyclopentenone prostaglandins protects against toxic effects of oxidized low-density lipoprotein

Linda Hamel, Margaret Kenney, Kenneth Clark, Linda Merkel, Camilo Rojas

Research output: Contribution to journalArticle

Abstract

Human vascular smooth muscle cells (SMC) exhibit upregulation of inducible heat shock protein 70 (Hsp70), upon exposure to oxidized low-density lipoproteins (LDL(ox)). The presence of Hsp70 is thought to protect the cell against the toxic effects of the modified lipoprotein. In order to test this hypothesis, Hsp70 in SMC was upregulated by exposure to Δ 12 prostaglandin J 212PGJ 2) before cells were exposed to LDL(ox). Hsp70 levels were measured after exposure to Δ 12PGJ 2 and before exposure to LDL(ox). Cell protection was monitored after LDL(ox) exposure by determination of cell toxicity measured by cell lactate dehydrogenase (LDH) release into the medium. Cells treated with Δ 12PGJ 2 exhibited a 23-fold increase in Hsp70 levels and 56% lower LDH activity release after exposure to LDL(ox) when compared to cells that were not pretreated with Δ 12PGJ 2. In addition, cells pretreated with prostaglandins that did not induce Hsp70 did not exhibit increased tolerance against the toxic effects of LDL(ox). The results support a protective role for Hsp70 against the toxic effects of LDL(ox) and hint at the potential for the use of small molecules for the prevention of deleterious effects of LDL(ox) through heat shock protein upregulation. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume269
Issue number2
DOIs
StatePublished - Mar 16 2000
Externally publishedYes

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Poisons
HSP70 Heat-Shock Proteins
Prostaglandins
Smooth Muscle Myocytes
Muscle
Cells
L-Lactate Dehydrogenase
Up-Regulation
cyclopentenone
oxidized low density lipoprotein
Cytoprotection
Heat-Shock Proteins
Vascular Smooth Muscle
Lipoproteins
Toxicity
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Preconditioning of human smooth muscle cells via cyclopentenone prostaglandins protects against toxic effects of oxidized low-density lipoprotein. / Hamel, Linda; Kenney, Margaret; Clark, Kenneth; Merkel, Linda; Rojas, Camilo.

In: Biochemical and Biophysical Research Communications, Vol. 269, No. 2, 16.03.2000, p. 327-330.

Research output: Contribution to journalArticle

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