Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging

Nathan Archer, Yu Wang, Roger V. Ortines, Haiyun Liu, Sabrina J. Nolan, Qi Liu, Martin P. Alphonse, Dustin A. Dikeman, Momina Mazhar, Robert J. Miller, Leif S. Anderson, Kevin P. Francis, Scott I. Simon, Lloyd S Miller

Research output: Contribution to journalArticle

Abstract

In vivo whole-animal optical (bioluminescence and fluorescence) imaging of Staphylococcus aureus infections has provided the opportunity to noninvasively and longitudinally monitor the dynamics of the bacterial burden and ensuing host immune responses in live anesthetized animals. Herein, we describe several different mouse models of S. aureus skin infection, skin inflammation, incisional/excisional wound infections, as well as mouse and rabbit models of orthopedic implant infection, which utilized this imaging technology. These animal models and imaging methodologies provide insights into the pathogenesis of these infections and innate and adaptive immune responses, as well as the preclinical evaluation of diagnostic and treatment modalities. Noninvasive approaches to investigate host-pathogen interactions are extremely important as virulent community-acquired methicillin-resistant S. aureus strains (CA-MRSA) are spreading through the normal human population, becoming more antibiotic resistant and creating a serious threat to public health.

Original languageEnglish (US)
Pages (from-to)197-228
Number of pages32
JournalMethods in molecular biology (Clifton, N.J.)
Volume2069
DOIs
StatePublished - Jan 1 2020

Fingerprint

Optical Imaging
Staphylococcus aureus
Infection
Host-Pathogen Interactions
Skin
Adaptive Immunity
Wound Infection
Methicillin-Resistant Staphylococcus aureus
Innate Immunity
Orthopedics
Animal Models
Public Health
Anti-Bacterial Agents
Rabbits
Inflammation
Technology
Population

Keywords

  • Biofilm
  • Bioluminescence
  • Bone
  • Fluorescence
  • Imaging
  • Immune
  • Implant
  • Infection
  • Inflammation
  • Optical
  • Orthopedic
  • Skin
  • Staphylococcus aureus
  • Wound

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging. / Archer, Nathan; Wang, Yu; Ortines, Roger V.; Liu, Haiyun; Nolan, Sabrina J.; Liu, Qi; Alphonse, Martin P.; Dikeman, Dustin A.; Mazhar, Momina; Miller, Robert J.; Anderson, Leif S.; Francis, Kevin P.; Simon, Scott I.; Miller, Lloyd S.

In: Methods in molecular biology (Clifton, N.J.), Vol. 2069, 01.01.2020, p. 197-228.

Research output: Contribution to journalArticle

Archer, N, Wang, Y, Ortines, RV, Liu, H, Nolan, SJ, Liu, Q, Alphonse, MP, Dikeman, DA, Mazhar, M, Miller, RJ, Anderson, LS, Francis, KP, Simon, SI & Miller, LS 2020, 'Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging', Methods in molecular biology (Clifton, N.J.), vol. 2069, pp. 197-228. https://doi.org/10.1007/978-1-4939-9849-4_15
Archer, Nathan ; Wang, Yu ; Ortines, Roger V. ; Liu, Haiyun ; Nolan, Sabrina J. ; Liu, Qi ; Alphonse, Martin P. ; Dikeman, Dustin A. ; Mazhar, Momina ; Miller, Robert J. ; Anderson, Leif S. ; Francis, Kevin P. ; Simon, Scott I. ; Miller, Lloyd S. / Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging. In: Methods in molecular biology (Clifton, N.J.). 2020 ; Vol. 2069. pp. 197-228.
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