Abstract
In vivo whole-animal optical (bioluminescence and fluorescence) imaging of Staphylococcus aureus infections has provided the opportunity to noninvasively and longitudinally monitor the dynamics of the bacterial burden and ensuing host immune responses in live anesthetized animals. Herein, we describe several different mouse models of S. aureus skin infection, skin inflammation, incisional/excisional wound infections, as well as mouse and rabbit models of orthopedic implant infection, which utilized this imaging technology. These animal models and imaging methodologies provide insights into the pathogenesis of these infections and innate and adaptive immune responses, as well as the preclinical evaluation of diagnostic and treatment modalities. Noninvasive approaches to investigate host-pathogen interactions are extremely important as virulent community-acquired methicillin-resistant S. aureus strains (CA-MRSA) are spreading through the normal human population, becoming more antibiotic resistant and creating a serious threat to public health.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 197-228 |
| Number of pages | 32 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 2069 |
| DOIs | |
| State | Published - Jan 1 2020 |
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Keywords
- Biofilm
- Bioluminescence
- Bone
- Fluorescence
- Imaging
- Immune
- Implant
- Infection
- Inflammation
- Optical
- Orthopedic
- Skin
- Staphylococcus aureus
- Wound
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging. / Archer, Nathan; Wang, Yu; Ortines, Roger V.; Liu, Haiyun; Nolan, Sabrina J.; Liu, Qi; Alphonse, Martin P.; Dikeman, Dustin A.; Mazhar, Momina; Miller, Robert J.; Anderson, Leif S.; Francis, Kevin P.; Simon, Scott I.; Miller, Lloyd S.
In: Methods in molecular biology (Clifton, N.J.), Vol. 2069, 01.01.2020, p. 197-228.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging
AU - Archer, Nathan
AU - Wang, Yu
AU - Ortines, Roger V.
AU - Liu, Haiyun
AU - Nolan, Sabrina J.
AU - Liu, Qi
AU - Alphonse, Martin P.
AU - Dikeman, Dustin A.
AU - Mazhar, Momina
AU - Miller, Robert J.
AU - Anderson, Leif S.
AU - Francis, Kevin P.
AU - Simon, Scott I.
AU - Miller, Lloyd S
PY - 2020/1/1
Y1 - 2020/1/1
N2 - In vivo whole-animal optical (bioluminescence and fluorescence) imaging of Staphylococcus aureus infections has provided the opportunity to noninvasively and longitudinally monitor the dynamics of the bacterial burden and ensuing host immune responses in live anesthetized animals. Herein, we describe several different mouse models of S. aureus skin infection, skin inflammation, incisional/excisional wound infections, as well as mouse and rabbit models of orthopedic implant infection, which utilized this imaging technology. These animal models and imaging methodologies provide insights into the pathogenesis of these infections and innate and adaptive immune responses, as well as the preclinical evaluation of diagnostic and treatment modalities. Noninvasive approaches to investigate host-pathogen interactions are extremely important as virulent community-acquired methicillin-resistant S. aureus strains (CA-MRSA) are spreading through the normal human population, becoming more antibiotic resistant and creating a serious threat to public health.
AB - In vivo whole-animal optical (bioluminescence and fluorescence) imaging of Staphylococcus aureus infections has provided the opportunity to noninvasively and longitudinally monitor the dynamics of the bacterial burden and ensuing host immune responses in live anesthetized animals. Herein, we describe several different mouse models of S. aureus skin infection, skin inflammation, incisional/excisional wound infections, as well as mouse and rabbit models of orthopedic implant infection, which utilized this imaging technology. These animal models and imaging methodologies provide insights into the pathogenesis of these infections and innate and adaptive immune responses, as well as the preclinical evaluation of diagnostic and treatment modalities. Noninvasive approaches to investigate host-pathogen interactions are extremely important as virulent community-acquired methicillin-resistant S. aureus strains (CA-MRSA) are spreading through the normal human population, becoming more antibiotic resistant and creating a serious threat to public health.
KW - Biofilm
KW - Bioluminescence
KW - Bone
KW - Fluorescence
KW - Imaging
KW - Immune
KW - Implant
KW - Infection
KW - Inflammation
KW - Optical
KW - Orthopedic
KW - Skin
KW - Staphylococcus aureus
KW - Wound
UR - http://www.scopus.com/inward/record.url?scp=85072216560&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072216560&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9849-4_15
DO - 10.1007/978-1-4939-9849-4_15
M3 - Article
C2 - 31523776
AN - SCOPUS:85072216560
VL - 2069
SP - 197
EP - 228
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -