TY - JOUR
T1 - Preclinical evaluation of photoacoustic imaging as a novel noninvasive approach to detect an orthopaedic implant infection
AU - Wang, Yu
AU - Thompson, John M.
AU - Ashbaugh, Alyssa G.
AU - Khodakivskyi, Pavlo
AU - Budin, Ghyslain
AU - Sinisi, Riccardo
AU - Heinmiller, Andrew
AU - Van Oosten, Marleen
AU - Van Dijl, Jan Maarten
AU - Van Dam, Gooitzen M.
AU - Francis, Kevin P.
AU - Bernthal, Nicholas M.
AU - Dubikovskaya, Elena A.
AU - Miller, Lloyd S.
N1 - Publisher Copyright:
© 2017 the American Academy of Orthopaedic Surgeons.
PY - 2017
Y1 - 2017
N2 - Introduction: Diagnosing prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for diagnosing PJI safely and noninvasively. Materials: In an established preclinical mouse model of bioluminescent Staphylococcus aureus PJI, fluorescent indocyanine green (ICG) was conjugated to b-cyclodextrin (CDX-ICG) or teicoplanin (Teic-ICG) and injected intravenously for 1 week postoperatively. Daily fluorescent imaging and PAI were used to localize and quantify tracer signals. Results were analyzed using 2-way analysis of variance. Results: Fluorescence clearly localized to the site of infection and was significantly higher with Teic-ICG compared with CDX-ICG (P = 0.046) and ICG alone (P = 0.0087). With PAI, the photoacoustic signal per volumetric analysis was substantially higher and better visualized with Teic-ICG compared with CDX-ICG and ICG alone, and colocalized well with bioluminescence and fluorescence imaging. Conclusion: Photoacoustic imaging successfully localized PJI in this proof-ofconcept study and demonstrates potential for clinical translation in orthopaedics.
AB - Introduction: Diagnosing prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for diagnosing PJI safely and noninvasively. Materials: In an established preclinical mouse model of bioluminescent Staphylococcus aureus PJI, fluorescent indocyanine green (ICG) was conjugated to b-cyclodextrin (CDX-ICG) or teicoplanin (Teic-ICG) and injected intravenously for 1 week postoperatively. Daily fluorescent imaging and PAI were used to localize and quantify tracer signals. Results were analyzed using 2-way analysis of variance. Results: Fluorescence clearly localized to the site of infection and was significantly higher with Teic-ICG compared with CDX-ICG (P = 0.046) and ICG alone (P = 0.0087). With PAI, the photoacoustic signal per volumetric analysis was substantially higher and better visualized with Teic-ICG compared with CDX-ICG and ICG alone, and colocalized well with bioluminescence and fluorescence imaging. Conclusion: Photoacoustic imaging successfully localized PJI in this proof-ofconcept study and demonstrates potential for clinical translation in orthopaedics.
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U2 - 10.5435/JAAOS-D-16-00630
DO - 10.5435/JAAOS-D-16-00630
M3 - Article
C2 - 27941556
AN - SCOPUS:85010660066
SN - 1067-151X
VL - 25
SP - S7-S12
JO - Journal of the American Academy of Orthopaedic Surgeons
JF - Journal of the American Academy of Orthopaedic Surgeons
ER -