Precardiac organoids form two heart fields via Bmp/Wnt signaling

Peter Andersen, Emmanouil Tampakakis, Dennisse V. Jimenez, Suraj Kannan, Matthew Miyamoto, Hye Kyung Shin, Amir Saberi, Sean Murphy, Edrick Sulistio, Stephen Chelko, Chulan Kwon

Research output: Contribution to journalArticle

Abstract

The discovery of the first heart field (FHF) and the second heart field (SHF) led us to understand how cardiac lineages and structures arise during development. However, it remains unknown how they are specified. Here, we generate precardiac spheroids with pluripotent stem cells (PSCs) harboring GFP/RFP reporters under the control of FHF/SHF markers, respectively. GFP+ cells and RFP+ cells appear from two distinct areas and develop in a complementary fashion. Transcriptome analysis shows a high degree of similarities with embryonic FHF/SHF cells. Bmp and Wnt are among the most differentially regulated pathways, and gain- and loss-of-function studies reveal that Bmp specifies GFP+ cells and RFP+ cells via the Bmp/Smad pathway and Wnt signaling, respectively. FHF/SHF cells can be isolated without reporters by the surface protein Cxcr4. This study provides novel insights into understanding the specification of two cardiac origins, which can be leveraged for PSC-based modeling of heart field/chamber-specific disease.

Original languageEnglish (US)
Article number3140
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Organoids
cells
Pluripotent Stem Cells
stem cells
Stem cells
Wnt Signaling Pathway
spheroids
Gene Expression Profiling
markers
specifications
Membrane Proteins
chambers
proteins
Specifications

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Precardiac organoids form two heart fields via Bmp/Wnt signaling. / Andersen, Peter; Tampakakis, Emmanouil; Jimenez, Dennisse V.; Kannan, Suraj; Miyamoto, Matthew; Shin, Hye Kyung; Saberi, Amir; Murphy, Sean; Sulistio, Edrick; Chelko, Stephen; Kwon, Chulan.

In: Nature Communications, Vol. 9, No. 1, 3140, 01.12.2018.

Research output: Contribution to journalArticle

Andersen, P, Tampakakis, E, Jimenez, DV, Kannan, S, Miyamoto, M, Shin, HK, Saberi, A, Murphy, S, Sulistio, E, Chelko, S & Kwon, C 2018, 'Precardiac organoids form two heart fields via Bmp/Wnt signaling', Nature Communications, vol. 9, no. 1, 3140. https://doi.org/10.1038/s41467-018-05604-8
Andersen, Peter ; Tampakakis, Emmanouil ; Jimenez, Dennisse V. ; Kannan, Suraj ; Miyamoto, Matthew ; Shin, Hye Kyung ; Saberi, Amir ; Murphy, Sean ; Sulistio, Edrick ; Chelko, Stephen ; Kwon, Chulan. / Precardiac organoids form two heart fields via Bmp/Wnt signaling. In: Nature Communications. 2018 ; Vol. 9, No. 1.
@article{c2623383b0184f09befba7b592228dcc,
title = "Precardiac organoids form two heart fields via Bmp/Wnt signaling",
abstract = "The discovery of the first heart field (FHF) and the second heart field (SHF) led us to understand how cardiac lineages and structures arise during development. However, it remains unknown how they are specified. Here, we generate precardiac spheroids with pluripotent stem cells (PSCs) harboring GFP/RFP reporters under the control of FHF/SHF markers, respectively. GFP+ cells and RFP+ cells appear from two distinct areas and develop in a complementary fashion. Transcriptome analysis shows a high degree of similarities with embryonic FHF/SHF cells. Bmp and Wnt are among the most differentially regulated pathways, and gain- and loss-of-function studies reveal that Bmp specifies GFP+ cells and RFP+ cells via the Bmp/Smad pathway and Wnt signaling, respectively. FHF/SHF cells can be isolated without reporters by the surface protein Cxcr4. This study provides novel insights into understanding the specification of two cardiac origins, which can be leveraged for PSC-based modeling of heart field/chamber-specific disease.",
author = "Peter Andersen and Emmanouil Tampakakis and Jimenez, {Dennisse V.} and Suraj Kannan and Matthew Miyamoto and Shin, {Hye Kyung} and Amir Saberi and Sean Murphy and Edrick Sulistio and Stephen Chelko and Chulan Kwon",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41467-018-05604-8",
language = "English (US)",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Precardiac organoids form two heart fields via Bmp/Wnt signaling

AU - Andersen, Peter

AU - Tampakakis, Emmanouil

AU - Jimenez, Dennisse V.

AU - Kannan, Suraj

AU - Miyamoto, Matthew

AU - Shin, Hye Kyung

AU - Saberi, Amir

AU - Murphy, Sean

AU - Sulistio, Edrick

AU - Chelko, Stephen

AU - Kwon, Chulan

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The discovery of the first heart field (FHF) and the second heart field (SHF) led us to understand how cardiac lineages and structures arise during development. However, it remains unknown how they are specified. Here, we generate precardiac spheroids with pluripotent stem cells (PSCs) harboring GFP/RFP reporters under the control of FHF/SHF markers, respectively. GFP+ cells and RFP+ cells appear from two distinct areas and develop in a complementary fashion. Transcriptome analysis shows a high degree of similarities with embryonic FHF/SHF cells. Bmp and Wnt are among the most differentially regulated pathways, and gain- and loss-of-function studies reveal that Bmp specifies GFP+ cells and RFP+ cells via the Bmp/Smad pathway and Wnt signaling, respectively. FHF/SHF cells can be isolated without reporters by the surface protein Cxcr4. This study provides novel insights into understanding the specification of two cardiac origins, which can be leveraged for PSC-based modeling of heart field/chamber-specific disease.

AB - The discovery of the first heart field (FHF) and the second heart field (SHF) led us to understand how cardiac lineages and structures arise during development. However, it remains unknown how they are specified. Here, we generate precardiac spheroids with pluripotent stem cells (PSCs) harboring GFP/RFP reporters under the control of FHF/SHF markers, respectively. GFP+ cells and RFP+ cells appear from two distinct areas and develop in a complementary fashion. Transcriptome analysis shows a high degree of similarities with embryonic FHF/SHF cells. Bmp and Wnt are among the most differentially regulated pathways, and gain- and loss-of-function studies reveal that Bmp specifies GFP+ cells and RFP+ cells via the Bmp/Smad pathway and Wnt signaling, respectively. FHF/SHF cells can be isolated without reporters by the surface protein Cxcr4. This study provides novel insights into understanding the specification of two cardiac origins, which can be leveraged for PSC-based modeling of heart field/chamber-specific disease.

UR - http://www.scopus.com/inward/record.url?scp=85051269777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051269777&partnerID=8YFLogxK

U2 - 10.1038/s41467-018-05604-8

DO - 10.1038/s41467-018-05604-8

M3 - Article

C2 - 30087351

AN - SCOPUS:85051269777

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3140

ER -