Pre-TCR-induced β-catenin facilitates traversal through β-selection

Mai Xu, Archna Sharma, David L. Wiest, Jyoti Misra Sen

Research output: Contribution to journalArticle

Abstract

Pre-TCR induced signals regulate development of the αβ TCR lineage cells at the β-selection checkpoint. We have previously shown that conditional deletion of β-catenin, a central mediator of Wnt-β-catenin-T cell factor signaling pathway, impairs traversal through the β-selection checkpoint. We now provide a molecular basis for the impairment. We demonstrate that pre-TCR signals specifically stabilize β-catenin in CD4-CD8- double negative thymocytes during β-selection. Pre-TCR induced Erk activity was required to stabilize β-catenin. Enforced expression of stabilized β-catenin was sufficient to mediate aspects of β-selection including sustained expression of early growth response (Egr) genes. Consistently, deletion of β-catenin reduced induction of Egr gene expression by the pre-TCR signal and blocked efficient β-selection. Thus, we demonstrate that pre-TCR induced β-catenin sustains expression of Egr genes that facilitate traversal through the β-selection checkpoint.

Original languageEnglish (US)
Pages (from-to)751-758
Number of pages8
JournalJournal of Immunology
Volume182
Issue number2
StatePublished - Jan 15 2009
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

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  • Cite this

    Xu, M., Sharma, A., Wiest, D. L., & Sen, J. M. (2009). Pre-TCR-induced β-catenin facilitates traversal through β-selection. Journal of Immunology, 182(2), 751-758.