Practical diagnostic testing for human immunodeficiency virus

J. B. Jackson, H. H. Balfour

Research output: Contribution to journalArticle

Abstract

Since the discovery of human immunodeficiency virus (HIV) as the causative agent of acquired immunodeficiency syndrome in 1983, there has been a proliferation of diagnostic tests. These assays can be used to detect the presence of HIV antibody, HIV antigen, HIV ribonucleic and deoxyribonucleic acids, and HIV reverse transcriptase. Enzyme-linked immunosorbent assays, Western blot, radioimmunoprecipitation assays, indirect immunofluorescence assays, reverse transcriptase assays, and several molecular hybridization techniques are currently available. Enzyme-linked immunosorbent, Western blot, and indirect immunofluorescence assays for HIV antibody are very sensitive, specific, and adaptable to most laboratories. An enzyme-linked immunosorbent assay for HIV antigen is also readily adaptable to most laboratories and will be commercially available soon. While the other assays are more tedious, they are valuable confirmatory tests and are suitable for reference laboratories. The biohazards of performing HIV testing can be minimized with proper biosafety measures.

Original languageEnglish (US)
Pages (from-to)124-138
Number of pages15
JournalClinical Microbiology Reviews
Volume1
Issue number1
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology

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  • Cite this

    Jackson, J. B., & Balfour, H. H. (1988). Practical diagnostic testing for human immunodeficiency virus. Clinical Microbiology Reviews, 1(1), 124-138.