Potentiation of IgE response in vitro by T cells from rats infected with Nippostrongylus brasiliensis

M. Suemura, K. Ishizaka

Research output: Contribution to journalArticle

Abstract

The effect of T cells from rats infected with Nippostrongylus brasiliensis (Nb) on the IgE response was studied by using an in vitro system. Mesenteric lymph node cells from DNP-OA (dinitrophenyl derivatives of ovalbumin)-primed rats were cultured with DNP-OA or DNP-HSA in the presence of T or B cells from Nb-infected rats. It was found that T cells from the infected rat, but not normal T cells, selectively enhanced the IgE-forming cell response of DNP-OA primed cells to homologous antigen without affecting the IgG-forming cell response. The enhancement of the IgE response requires carrier primed T cells; T cells from Nb-infected animals failed to enhance the Ig-forming cell response of DNP-OA primed cells to DNP-HSA. Culture supernatants of T cells from Nb-infected rats selectively enhanced the IgE response of DNP-OA primed cells to homologous antigen without affecting IgG response. The IgE-potentiating factor present in the unstimulated cultures is different from T cell-replacing factor(s), which was obtained by stimulation of antigen-primed T cells with antigen. When T cells from Nb-infected rats were incubated with Nb antigen, culture supernatant of the cells enhanced both IgE and IgG responses of DNP-OA primed cells to DNP-OA or DNP-HSA. It was also found that the molecular size of IgE-potentiating factor was significantly smaller than T cell-replacing factor. The results suggested that an optimal IgE response may require two subsets of T cells; one is carrier-specific T cells, which are common for all isotypes, and another subset is cells forming IgE-potentiating factor.

Original languageEnglish (US)
Pages (from-to)918-924
Number of pages7
JournalJournal of Immunology
Volume123
Issue number2
StatePublished - 1979

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Nippostrongylus
Immunoglobulin E
Ovalbumin
T-Lymphocytes
Antigens
Immunoglobulin G
Interleukin-5
In Vitro Techniques
T-Lymphocyte Subsets
B-Lymphocytes
Cell Culture Techniques
Lymph Nodes

ASJC Scopus subject areas

  • Immunology

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Potentiation of IgE response in vitro by T cells from rats infected with Nippostrongylus brasiliensis. / Suemura, M.; Ishizaka, K.

In: Journal of Immunology, Vol. 123, No. 2, 1979, p. 918-924.

Research output: Contribution to journalArticle

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