Potential role of cervicovaginal extracellular particles in diagnosis of endometriosis

Dillon C. Muth, Melissa A. McAlexander, Lauren J. Ostrenga, Nathan M. Pate, Jessica Izzi, Robert John Adams, Kelly Pate, Sarah Beck, Baktiar O. Karim, Kenneth Whitaker Witwer

Research output: Contribution to journalArticle

Abstract

Background: Macaques are an excellent model for many human diseases, including reproductive diseases such as endometriosis. A long-recognized need for early biomarkers of endometriosis has not yet resulted in consensus. While biomarker studies have examined many bodily fluids and targets, cervicovaginal secretions have been relatively under-investigated. Extracellular vesicles (EVs, including exosomes and microvesicles) are found in every biofluid examined, carry cargo including proteins and RNA, and may participate in intercellular signaling. Little is known about EVs in the cervicovaginal compartment, including the effects of reproductive tract disease on quantity and quality of EVs. Case presentation: In September 2014, a 9-year-old rhesus macaque was diagnosed with endometriosis at The Johns Hopkins University School of Medicine. Ultrasound-guided fine needle aspiration of a cyst and subsequent laparotomy confirmed diagnosis. The animal was sent to necropsy following euthanasia for humane reasons. Perimortem vaginal swabs and cervicovaginal lavages were obtained. Using a combination of methods, including ultracentrifugation and NanoSight visualization technology, approximate numbers of EVs from each sample were calculated and compared to populations of EVs from other, reproductively normal macaques. Fewer EVs were recovered from the endometriosis samples as compared with those from reproductively healthy individuals. Conclusion: To our knowledge, this is the first examination of EVs in primate cervicovaginal secretions, including those of a macaque with endometriosis. This case study suggests that additional research is justified to determine whether quantification of EVs-or their molecular cargo-in cervicovaginal lavage and vaginal swabs may provide a novel, relatively non-invasive diagnostic for primate endometrial disease or other reproductive tract diseases.

Original languageEnglish (US)
Article number187
JournalBMC Veterinary Research
Volume11
Issue number1
DOIs
StatePublished - Aug 8 2015

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exosomes
Exosomes
Endometriosis
Macaca
Primate Diseases
biomarkers
Primates
Vaginal Douching
Biomarkers
Uterine Diseases
secretion
reproductive disorders
Euthanasia
ultracentrifugation
Therapeutic Irrigation
Ultracentrifugation
laparotomy
euthanasia
Fine Needle Biopsy
Macaca mulatta

ASJC Scopus subject areas

  • veterinary(all)

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Potential role of cervicovaginal extracellular particles in diagnosis of endometriosis. / Muth, Dillon C.; McAlexander, Melissa A.; Ostrenga, Lauren J.; Pate, Nathan M.; Izzi, Jessica; Adams, Robert John; Pate, Kelly; Beck, Sarah; Karim, Baktiar O.; Witwer, Kenneth Whitaker.

In: BMC Veterinary Research, Vol. 11, No. 1, 187, 08.08.2015.

Research output: Contribution to journalArticle

Muth, Dillon C. ; McAlexander, Melissa A. ; Ostrenga, Lauren J. ; Pate, Nathan M. ; Izzi, Jessica ; Adams, Robert John ; Pate, Kelly ; Beck, Sarah ; Karim, Baktiar O. ; Witwer, Kenneth Whitaker. / Potential role of cervicovaginal extracellular particles in diagnosis of endometriosis. In: BMC Veterinary Research. 2015 ; Vol. 11, No. 1.
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