Potential problems in the use of commercial preparations of radiolabeled cholesterol

Florence H. Mahlberg, Annabelle Rodriguez-Oquendo, David W. Bernard, Jane M. Glick, George H. Rothblat

Research output: Contribution to journalArticle

Abstract

Through a series of biological and analytical procedures, we demonstrate that a compound purchased from a commercial supplier as [7-3H]cholesterol was not cholesterol. In mouse peritoneal macrophages, this compound was metabolized differently than other radiolabeled cholesterol preparations and was accumulated in the steryl ester pool. In contrast, Fu5AH rat hepatoma cells did not discriminate this compound from cholesterol. Further analysis of the anomalous [7-3H]cholesterol by TLC after cholesterol oxidase treatment and by HPLC indicated that this radiochemical was less polar than cholesterol standard and other radiolabeled cholesterol preparations tested. Mass spectrometry analysis disclosed that the chemical has a similar fragmentation pattern avnd the same molecular weight (386) as cholesterol.

Original languageEnglish (US)
Pages (from-to)95-100
Number of pages6
JournalAtherosclerosis
Volume84
Issue number2-3
DOIs
StatePublished - 1990
Externally publishedYes

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Cholesterol
Cholesterol Oxidase
Peritoneal Macrophages
Hepatocellular Carcinoma
Mass Spectrometry
Esters
Molecular Weight
High Pressure Liquid Chromatography

Keywords

  • Cholesterol
  • Commercial cholesterol preparations
  • Radiolabeled cholesterol

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Potential problems in the use of commercial preparations of radiolabeled cholesterol. / Mahlberg, Florence H.; Rodriguez-Oquendo, Annabelle; Bernard, David W.; Glick, Jane M.; Rothblat, George H.

In: Atherosclerosis, Vol. 84, No. 2-3, 1990, p. 95-100.

Research output: Contribution to journalArticle

Mahlberg, Florence H. ; Rodriguez-Oquendo, Annabelle ; Bernard, David W. ; Glick, Jane M. ; Rothblat, George H. / Potential problems in the use of commercial preparations of radiolabeled cholesterol. In: Atherosclerosis. 1990 ; Vol. 84, No. 2-3. pp. 95-100.
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