Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus

G. Mani Subramanian; Paul A. Moore; Brian B. Gowen; Aaron L. Olsen; Dale L. Barnard; Jason Paragas; Robert J. Hogan; Robert W. Sidwell

doi:10.1159/000140361

Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus

G. Mani Subramanian, Paul A. Moore, Brian B. Gowen, Aaron L. Olsen, Dale L. Barnard, Jason Paragas, Robert J. Hogan, Robert W. Sidwell

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Background: The type 1 interferons (INF-α and INF-β) are potent antiviral agents. Albumin-INF-α and albumin-INF-β are novel recombinant proteins consisting of IFN-α or IFN-β genetically fused to human albumin. Methods: The in vitro antiviral activity of albumin-IFN-α was evaluated against representative bioterrorism viral agents and the severe acute respiratory syndrome virus. Antiviral activity was assessed using inhibition of cytopathic effect and neutral red staining. Results: EC₅₀ values for albumin-IFN-α ranged from <0.1 ng/ml for Punta Toro virus to 65 ng/ml for Venezuelan equine encephalitis virus in the neutral red assay. Albumin-IFN-β showed 75- and 360-fold greater in vitro activity than albumin-IFN-α against Ebola virus and severe acute respiratory syndrome, respectively. Conclusion: Further evaluation of these long-acting albumin-IFN fusion proteins as prophylactic or therapeutic agents against these viral agents of bioterrorism in relevant primate models is warranted.

Original language	English (US)
Pages (from-to)	176-180
Number of pages	5
Journal	CHEMOTHERAPY
Volume	54
Issue number	3
DOIs	https://doi.org/10.1159/000140361
State	Published - Jul 2008
Externally published	Yes

Keywords

Albumin-interferon-α
Albumin-interferon-β
Bioterrorism
RNA virus

ASJC Scopus subject areas

Oncology
Pharmacology
Drug Discovery
Pharmacology (medical)
Infectious Diseases

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Subramanian, G. M., Moore, P. A., Gowen, B. B., Olsen, A. L., Barnard, D. L., Paragas, J., Hogan, R. J., & Sidwell, R. W. (2008). Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus. CHEMOTHERAPY, 54(3), 176-180. https://doi.org/10.1159/000140361

Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus. / Subramanian, G. Mani; Moore, Paul A.; Gowen, Brian B. et al.
In: CHEMOTHERAPY, Vol. 54, No. 3, 07.2008, p. 176-180.

Research output: Contribution to journal › Article › peer-review

Subramanian, GM, Moore, PA, Gowen, BB, Olsen, AL, Barnard, DL, Paragas, J, Hogan, RJ & Sidwell, RW 2008, 'Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus', CHEMOTHERAPY, vol. 54, no. 3, pp. 176-180. https://doi.org/10.1159/000140361

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abstract = "Background: The type 1 interferons (INF-α and INF-β) are potent antiviral agents. Albumin-INF-α and albumin-INF-β are novel recombinant proteins consisting of IFN-α or IFN-β genetically fused to human albumin. Methods: The in vitro antiviral activity of albumin-IFN-α was evaluated against representative bioterrorism viral agents and the severe acute respiratory syndrome virus. Antiviral activity was assessed using inhibition of cytopathic effect and neutral red staining. Results: EC50 values for albumin-IFN-α ranged from <0.1 ng/ml for Punta Toro virus to 65 ng/ml for Venezuelan equine encephalitis virus in the neutral red assay. Albumin-IFN-β showed 75- and 360-fold greater in vitro activity than albumin-IFN-α against Ebola virus and severe acute respiratory syndrome, respectively. Conclusion: Further evaluation of these long-acting albumin-IFN fusion proteins as prophylactic or therapeutic agents against these viral agents of bioterrorism in relevant primate models is warranted.",

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AU - Subramanian, G. Mani

AU - Moore, Paul A.

AU - Gowen, Brian B.

AU - Olsen, Aaron L.

AU - Barnard, Dale L.

AU - Paragas, Jason

AU - Hogan, Robert J.

AU - Sidwell, Robert W.

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N2 - Background: The type 1 interferons (INF-α and INF-β) are potent antiviral agents. Albumin-INF-α and albumin-INF-β are novel recombinant proteins consisting of IFN-α or IFN-β genetically fused to human albumin. Methods: The in vitro antiviral activity of albumin-IFN-α was evaluated against representative bioterrorism viral agents and the severe acute respiratory syndrome virus. Antiviral activity was assessed using inhibition of cytopathic effect and neutral red staining. Results: EC50 values for albumin-IFN-α ranged from <0.1 ng/ml for Punta Toro virus to 65 ng/ml for Venezuelan equine encephalitis virus in the neutral red assay. Albumin-IFN-β showed 75- and 360-fold greater in vitro activity than albumin-IFN-α against Ebola virus and severe acute respiratory syndrome, respectively. Conclusion: Further evaluation of these long-acting albumin-IFN fusion proteins as prophylactic or therapeutic agents against these viral agents of bioterrorism in relevant primate models is warranted.

AB - Background: The type 1 interferons (INF-α and INF-β) are potent antiviral agents. Albumin-INF-α and albumin-INF-β are novel recombinant proteins consisting of IFN-α or IFN-β genetically fused to human albumin. Methods: The in vitro antiviral activity of albumin-IFN-α was evaluated against representative bioterrorism viral agents and the severe acute respiratory syndrome virus. Antiviral activity was assessed using inhibition of cytopathic effect and neutral red staining. Results: EC50 values for albumin-IFN-α ranged from <0.1 ng/ml for Punta Toro virus to 65 ng/ml for Venezuelan equine encephalitis virus in the neutral red assay. Albumin-IFN-β showed 75- and 360-fold greater in vitro activity than albumin-IFN-α against Ebola virus and severe acute respiratory syndrome, respectively. Conclusion: Further evaluation of these long-acting albumin-IFN fusion proteins as prophylactic or therapeutic agents against these viral agents of bioterrorism in relevant primate models is warranted.

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Potent in vitro activity of the albumin fusion type 1 interferons (albumin-interferon-alpha and albumin-interferon-beta) against RNA viral agents of bioterrorism and the severe acute respiratory syndrome (SARS) virus

Abstract

Keywords

ASJC Scopus subject areas

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