Potassium transport in the early distal tubule of Amphiuma kidney - Effects of potassium adaptation

Hans Oberleithner, William Guggino, Gerhard Giebisch

Research output: Contribution to journalArticle

Abstract

Studies were performed to investigate potassium transport in early distal tubule of the doubly-perfused kidney of Amphiuma under control conditions and following K-adaptation. Double barreled K-sensitive microelectrodes were used in stationary microperfusion experiments. Net K-flux was evaluated along with measurements of both cell membrane potential and cell K activity. Net K flux and electrochemical driving forces of K were described over a wide range of peritubular K concentrations. Whereas in control animals, at normal and low peritubular K concentrations K reabsorption occurs, K secretion is induced by elevating peritubular K. In contrast, net K secretion is seen at all peritubular K levels in the K-adapted kidney. Net K secretion approaches saturation at high peritubular K concentrations. Intracellular K activities also approach plateau values which are shifted upward in the state of K-adaptation. In control animals at zero net flux conditions intracellular K is maintained above electrochemical equilibrium across both the peritubular and the luminal cell membrane. After K-adaptation, however, K approaches electrochemical equilibrium across the luminal cell membrane. The results indicate that in control conditions, K is taken up actively into the cell across the peritubular and across the luminal cell barrier. It is likely that both luminal and peritubular transport components (increased luminal K conductance, diminished luminal K cotransport, stimulation of peritubular K-uptake) are responsible for increased K secretion during K adaptation.

Original languageEnglish (US)
Pages (from-to)185-191
Number of pages7
JournalPflügers Archiv European Journal of Physiology
Volume396
Issue number3
DOIs
StatePublished - Mar 1 1983
Externally publishedYes

Keywords

  • Amphibian kidney
  • Early distal tubule
  • Potassium adaptation
  • Potassium sensitive microelectrodes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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