Posttreatment follow-up of Helicobacter pylori infection using a stool antigen immunoassay

D. E. Roth, D. N. Taylor, Robert H Gilman, R. Meza, U. Katz, C. Bautista, L. Cabrera, B. Velapatiño, C. Lebron, M. Razúri, J. Watanabe, T. Monath

Research output: Contribution to journalArticle

Abstract

The Helicobacter pylori stool antigen enzyme immunoassay (HpSA) was evaluated during posttreatment follow-up of patients in a country with a very high prevalence of H. pylori infection. From among 273 dyspeptic individuals (18 to 55 years) initially recruited from a shantytown in Lima, Peru, 238 participants who met the inclusion criteria and were suspected to be H. pylori positive based on 14C urea breath test (UBT) results underwent endoscopy. Participants with endoscopy-proven infections received standard eradication therapy and were monitored by UBT and HpSA at 1 month following treatment and at 3-month intervals for 9 months posttreatment. A second endoscopy was performed if UBT results showed evidence of treatment failure or H. priori recurrence. Biopsy results were considered the "gold standard" in all analyses. Among patients who underwent endoscopy, HpSA had a pretreatment sensitivity of 93%. TWo-hundred thirty patients completed the treatment regimen, of whom 201 (93%) were considered to have had successful treatment outcomes based on a negative follow-up UBT. Thirty-two patients with UBT-defined treatment failures or H. pylori recurrences at any point during the 9-month follow-up underwent a second endoscopy. In the posttreatment setting, HpSA had an overall sensitivity of 73% and a specificity of 67%. Agreement between UBT and HpSA diminished throughout the follow-up. Among 14 participants in whom HpSA remained positive at 1 month following treatment despite UBT evidence of treatment success, 12 (86%) became HpSA negative within 3 months posttreatment. Although this study confirmed the validity of the HpSA in the initial assessment of dyspeptic patients, the test demonstrated a reduced overall accuracy in the detection of treatment failures and H. pylori recurrences during 9 months of posttreatment follow-up. Furthermore, in some patients it may take up to 3 months after successful eradication for antigen shedding to diminish to levels within the negative HpSA range.

Original languageEnglish (US)
Pages (from-to)718-723
Number of pages6
JournalClinical and Diagnostic Laboratory Immunology
Volume8
Issue number4
DOIs
StatePublished - 2001

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Helicobacter Infections
Immunoassay
Helicobacter pylori
Immunoenzyme Techniques
Antigens
Breath Tests
Urea
Endoscopy
Enzymes
Treatment Failure
Recurrence
Biopsy
Therapeutics
Peru

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Posttreatment follow-up of Helicobacter pylori infection using a stool antigen immunoassay. / Roth, D. E.; Taylor, D. N.; Gilman, Robert H; Meza, R.; Katz, U.; Bautista, C.; Cabrera, L.; Velapatiño, B.; Lebron, C.; Razúri, M.; Watanabe, J.; Monath, T.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 8, No. 4, 2001, p. 718-723.

Research output: Contribution to journalArticle

Roth, DE, Taylor, DN, Gilman, RH, Meza, R, Katz, U, Bautista, C, Cabrera, L, Velapatiño, B, Lebron, C, Razúri, M, Watanabe, J & Monath, T 2001, 'Posttreatment follow-up of Helicobacter pylori infection using a stool antigen immunoassay', Clinical and Diagnostic Laboratory Immunology, vol. 8, no. 4, pp. 718-723. https://doi.org/10.1128/CDLI.8.4.718-723.2001
Roth, D. E. ; Taylor, D. N. ; Gilman, Robert H ; Meza, R. ; Katz, U. ; Bautista, C. ; Cabrera, L. ; Velapatiño, B. ; Lebron, C. ; Razúri, M. ; Watanabe, J. ; Monath, T. / Posttreatment follow-up of Helicobacter pylori infection using a stool antigen immunoassay. In: Clinical and Diagnostic Laboratory Immunology. 2001 ; Vol. 8, No. 4. pp. 718-723.
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