Posttranslational processing of the thrombopoietin receptor is impaired in polycythemia vera

Research output: Contribution to journalArticle

Abstract

Recently, we demonstrated a marked reduction in the expression of the thrombopoietin receptor, Mpl, in polycythemia vera (PV) platelets and megakaryocytes using an antiserum against the Mpl extracellular domain. To further examine this abnormality, we raised an antibody to the Mpl C- terminus. Immunologic analysis of PV platelets with this antiserum confirmed the reduction in Mpl expression. However, the C-terminal antiserum detected 2 forms of Mpl in PV platelets in contrast to normal platelets, in which a single form of Mpl was detected by both the extracellular domain and C- terminal antisera. Two-dimensional gel electrophoresis studies with isoelectric focusing in the first dimension identified normal platelet Mpl as an 85 to 92 kD protein with an isoelectric point (pl) of 5.5. PV platelets contained an additional 80 to 82 kD Mpl Mpl isoform with a pl of 6.5. Analysis of Mpl expressed by the human megakaryocytic cell line, Dami, showed 2 isoforms similar to those found in PV platelets suggesting a precursor- product relationship. Digestion of Dami cell and normal platelet lysates with neuraminidase converted the more acidic Mpl isoform to the more basic one, indicating that the 2 isoforms differed with respect to posttranslational glycosylation. Furthermore, in contrast to normal platelet Mpl, PV platelet Mpl was susceptible to endoglycosidase H digestion, indicating defective Mpl processing by PV megakaryocytes. The glycosylation defect was specific for Mpl, as 2 other platelet membrane glycoproteins, glycoprotein IIb and multimerin, were processed normally. Importantly, the extent of the PV platelet Mpl glycosylation defect correlated with disease duration and extramedullary hematopoiesis.

Original languageEnglish (US)
Pages (from-to)2555-2561
Number of pages7
JournalBlood
Volume94
Issue number8
StatePublished - Oct 15 1999

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Thrombopoietin Receptors
Polycythemia Vera
Platelets
Blood Platelets
Processing
Glycosylation
Immune Sera
Protein Isoforms
Megakaryocytes
Digestion
Extramedullary Hematopoiesis
Platelet Membrane Glycoproteins
Defects
Glycoside Hydrolases
Electrophoresis, Gel, Two-Dimensional
Isoelectric Point
Neuraminidase
Isoelectric Focusing
Electrophoresis

ASJC Scopus subject areas

  • Hematology

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Posttranslational processing of the thrombopoietin receptor is impaired in polycythemia vera. / Moliterno, Alison R; Spivak, Jerry L.

In: Blood, Vol. 94, No. 8, 15.10.1999, p. 2555-2561.

Research output: Contribution to journalArticle

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