Postnatal growth and behavioral development of mice cloned from adult cumulus cells

Kellie Tamashiro, Teruhiko Wakayama, Robert J. Blanchard, D. Caroline Blanchard, Ryuzo Yanagimachi

Research output: Contribution to journalArticle

Abstract

Since the first successful cloning of mammals from adult somatic cells, there has been no examination of the learning or behavior of cloned offspring. The possibility of adverse effects on animals produced through adult somatic cell cloning is high because many natural biological processes are bypassed and DNA from adult cells, which presumably contain mutations, are used. In this study, we compared cloned mice produced by microinjection transfer of cumulus cell nuclei into enucleated oocytes, to control mice that were specifically generated to eliminate confounding factors that are unique to our cloning procedure. Postnatal weight gain of clones was significantly greater than that of controls. Preweaning development observations revealed that first appearance or performance of 3 out of 10 measures was delayed in cloned mice; however, results of subsequent tests of learning and memory, activity level, and motor skills were comparable for both groups. Together, these data suggest that nuclear transfer of adult somatic cell nuclei to produce cloned mice may delay the appearance of a few developmental milestones but it does not adversely affect the overall postnatal behavior of mice. In addition, this procedure may cause late onset of significantly increased body weight in cloned offspring, the cause or causes of which are being further examined.

Original languageEnglish (US)
Pages (from-to)328-334
Number of pages7
JournalBiology of Reproduction
Volume63
Issue number1
StatePublished - 2000
Externally publishedYes

Fingerprint

Cumulus Cells
Growth and Development
Organism Cloning
Cell Nucleus
Learning
Biological Phenomena
Motor Skills
Microinjections
Weight Gain
Oocytes
Mammals
Clone Cells
Body Weight
Mutation
DNA

Keywords

  • Aging
  • Cumulus cells
  • Developmental biology

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Tamashiro, K., Wakayama, T., Blanchard, R. J., Blanchard, D. C., & Yanagimachi, R. (2000). Postnatal growth and behavioral development of mice cloned from adult cumulus cells. Biology of Reproduction, 63(1), 328-334.

Postnatal growth and behavioral development of mice cloned from adult cumulus cells. / Tamashiro, Kellie; Wakayama, Teruhiko; Blanchard, Robert J.; Blanchard, D. Caroline; Yanagimachi, Ryuzo.

In: Biology of Reproduction, Vol. 63, No. 1, 2000, p. 328-334.

Research output: Contribution to journalArticle

Tamashiro, K, Wakayama, T, Blanchard, RJ, Blanchard, DC & Yanagimachi, R 2000, 'Postnatal growth and behavioral development of mice cloned from adult cumulus cells', Biology of Reproduction, vol. 63, no. 1, pp. 328-334.
Tamashiro K, Wakayama T, Blanchard RJ, Blanchard DC, Yanagimachi R. Postnatal growth and behavioral development of mice cloned from adult cumulus cells. Biology of Reproduction. 2000;63(1):328-334.
Tamashiro, Kellie ; Wakayama, Teruhiko ; Blanchard, Robert J. ; Blanchard, D. Caroline ; Yanagimachi, Ryuzo. / Postnatal growth and behavioral development of mice cloned from adult cumulus cells. In: Biology of Reproduction. 2000 ; Vol. 63, No. 1. pp. 328-334.
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