Postnatal development of corticosteroid receptor immunoreactivity in the rat hippocampus

A. Lawson, R. Ahima, Z. Krozowski, R. Harlan

Research output: Contribution to journalArticlepeer-review

Abstract

Postnatal changes in corticosteroid receptor immunoreactivity in the rat hippocampus were examined using an antiserum against a fusion protein containing an N-terminal peptide of the Type I receptor, and a monoclonal antibody against the rat liver Type II-receptor. Age-related regional differences were observed. In the pyramidal cell layer of Ammon's horn, and granule cell layer of the dentate gyrus (DG), the percentage of Type I receptor immunoreactive (Type I-ir) and Type II receptor immunoreactive (Type II-ir) cells was high perinatally, declined sharply by postnatal day 10 (P10), and showed a variable increase to adult levels subsequently. The pyramidal cells of CA1-CA2, subiculum and DG showed a selective increase in Type II-ir in late postnatal life into adulthood, while most other regions showed higher Type I-ir in both early and late postnatal life, suggesting different roles for these receptors during development. Type II-ir was predominantly nuclear in most neurons, except for a transient appearance of cytoplasmic Type II-ir in neurons of the stratum oriens and molecular layers of Ammon's horn and dentate gyrus of P20-P30. Type I-ir was diffusely nuclear and cytoplasmic at all developmental ages. This is suggestive of differential genomic and extragenomic roles for these receptors during postnatal development of the hippocampus.

Original languageEnglish (US)
Pages (from-to)69-79
Number of pages11
JournalDevelopmental Brain Research
Volume62
Issue number1
DOIs
StatePublished - Sep 19 1991
Externally publishedYes

Keywords

  • Hippocampus
  • Immunoreactivity
  • Rat
  • Type I receptor
  • Type II receptor

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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