Postischemic cerebrovascular E-selectin expression mediates tissue injury in murine stroke

Judy Huang, Tanvir F. Choudhri, Christopher J. Winfree, Ryan A. McTaggart, Szilard Kiss, J. Mocco, Louis J. Kim, Themistocles S. Protopsaltis, Yuan Zhang, David J. Pinsky, E. Sander Connolly

Research output: Contribution to journalArticle

Abstract

Background and Purpose - Although the deleterious role of several proinflammatory mediators, including P-selectin, in reperfused stroke is well established, the role of E-selectin has not been fully characterized. Methods - E-selectin mRNA expression was studied at 4, 10, and 24 hours after reperfusion with reverse transcription and polymerase chain reaction in mice (n=18) subjected to transient intraluminal middle cerebral artery occlusion (MCAO). Mice received intravenous injection with anti-E-selectin monoclonal antibody (10, 35, or 50 μg), nonimmune IgG, or vehicle immediately before MCAO and 90 minutes later (n=85). Others received anti-E-selectin antibody 3 or 6 hours after MCAO (n=32). Myeloperoxidase activity was measured in sham-operated mice and after 10 hours of reperfusion in saline-, nonimmune IgG-, or anti-E-selectin IgG-treated cohorts (n=17). Serial cerebral blood flow was measured with laser-Doppler flowmetry, and outcomes were assessed by neurological deficits and infarct volumes with the use of planimetric analysis of triphenyltetrazolium chloride-stained sections. Results - Upregulated E-selectin expression occurred in the ischemic cerebral vasculature within 4 hours of reperfusion and persisted for 24 hours. Anti-E-selectin antibody increased ischemic cortical cerebral blood flow up to 2.6-fold (P

Original languageEnglish (US)
Pages (from-to)3047-3053
Number of pages7
JournalStroke
Volume31
Issue number12
StatePublished - 2000
Externally publishedYes

Fingerprint

E-Selectin
Stroke
Wounds and Injuries
Middle Cerebral Artery Infarction
Cerebrovascular Circulation
Reperfusion
Immunoglobulin G
Laser-Doppler Flowmetry
P-Selectin
Antibodies
Intravenous Injections
Peroxidase
Reverse Transcription
Monoclonal Antibodies
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cerebral ischemia, focal
  • Cerebral ischemia, transient
  • E-selectin
  • Gene expression
  • Mice

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Huang, J., Choudhri, T. F., Winfree, C. J., McTaggart, R. A., Kiss, S., Mocco, J., ... Connolly, E. S. (2000). Postischemic cerebrovascular E-selectin expression mediates tissue injury in murine stroke. Stroke, 31(12), 3047-3053.

Postischemic cerebrovascular E-selectin expression mediates tissue injury in murine stroke. / Huang, Judy; Choudhri, Tanvir F.; Winfree, Christopher J.; McTaggart, Ryan A.; Kiss, Szilard; Mocco, J.; Kim, Louis J.; Protopsaltis, Themistocles S.; Zhang, Yuan; Pinsky, David J.; Connolly, E. Sander.

In: Stroke, Vol. 31, No. 12, 2000, p. 3047-3053.

Research output: Contribution to journalArticle

Huang, J, Choudhri, TF, Winfree, CJ, McTaggart, RA, Kiss, S, Mocco, J, Kim, LJ, Protopsaltis, TS, Zhang, Y, Pinsky, DJ & Connolly, ES 2000, 'Postischemic cerebrovascular E-selectin expression mediates tissue injury in murine stroke', Stroke, vol. 31, no. 12, pp. 3047-3053.
Huang J, Choudhri TF, Winfree CJ, McTaggart RA, Kiss S, Mocco J et al. Postischemic cerebrovascular E-selectin expression mediates tissue injury in murine stroke. Stroke. 2000;31(12):3047-3053.
Huang, Judy ; Choudhri, Tanvir F. ; Winfree, Christopher J. ; McTaggart, Ryan A. ; Kiss, Szilard ; Mocco, J. ; Kim, Louis J. ; Protopsaltis, Themistocles S. ; Zhang, Yuan ; Pinsky, David J. ; Connolly, E. Sander. / Postischemic cerebrovascular E-selectin expression mediates tissue injury in murine stroke. In: Stroke. 2000 ; Vol. 31, No. 12. pp. 3047-3053.
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AU - Kiss, Szilard

AU - Mocco, J.

AU - Kim, Louis J.

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AU - Pinsky, David J.

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