Postinjection kinetics of antepartum Rh immune globulin

Frank R Witter, Rosetta S. Shirey, Suzy L. Nicol, Paul Michael Ness

Research output: Contribution to journalArticle

Abstract

The disappearance kinetics of a standard dose of 300 μg of Rh immune globulin given at 28 weeks' gestation was investigated. Ten Rh-negative unsensitized women were given 300 μg of Rh immune globulin at 28 weeks and blood samples were drawn at weekly intervals until delivery. Sera were titrated with Ror red blood cells in a saline solution-antiglobulin method. Titers ranged from 1 to 4 by the first week after injection. In nine of 10 patients the titers were zero at 49 to 70 days after injection. With R2R2 red blood cells and LISS, papain, polybrene, or a combination of methods, Rh immune globulin could still be detected until delivery in four of the nine patients. Because these serologic methods can detect 5 ng/ml anti-D, five of 10 subjects may not have been protected for 8 to 29 days before delivery. This study indicates that a titer of ≤4 is expected for passively acquired anti-D during pregnancy, and that antepartum administration of Rh immune globulin may not always produce detectable passive antibody for as long as theoretically predicted.

Original languageEnglish (US)
Pages (from-to)784-786
Number of pages3
JournalAmerican Journal of Obstetrics and Gynecology
Volume163
Issue number3
DOIs
StatePublished - 1990

Fingerprint

Rho(D) Immune Globulin
Erythrocytes
Hexadimethrine Bromide
Pregnancy
Injections
Papain
Sodium Chloride
Anti-Idiotypic Antibodies
Antibodies
Serum

Keywords

  • erythroblastosis fetalis
  • Rh disease
  • Rh hemolytic disease
  • Rh sensitization

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Postinjection kinetics of antepartum Rh immune globulin. / Witter, Frank R; Shirey, Rosetta S.; Nicol, Suzy L.; Ness, Paul Michael.

In: American Journal of Obstetrics and Gynecology, Vol. 163, No. 3, 1990, p. 784-786.

Research output: Contribution to journalArticle

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N2 - The disappearance kinetics of a standard dose of 300 μg of Rh immune globulin given at 28 weeks' gestation was investigated. Ten Rh-negative unsensitized women were given 300 μg of Rh immune globulin at 28 weeks and blood samples were drawn at weekly intervals until delivery. Sera were titrated with Ror red blood cells in a saline solution-antiglobulin method. Titers ranged from 1 to 4 by the first week after injection. In nine of 10 patients the titers were zero at 49 to 70 days after injection. With R2R2 red blood cells and LISS, papain, polybrene, or a combination of methods, Rh immune globulin could still be detected until delivery in four of the nine patients. Because these serologic methods can detect 5 ng/ml anti-D, five of 10 subjects may not have been protected for 8 to 29 days before delivery. This study indicates that a titer of ≤4 is expected for passively acquired anti-D during pregnancy, and that antepartum administration of Rh immune globulin may not always produce detectable passive antibody for as long as theoretically predicted.

AB - The disappearance kinetics of a standard dose of 300 μg of Rh immune globulin given at 28 weeks' gestation was investigated. Ten Rh-negative unsensitized women were given 300 μg of Rh immune globulin at 28 weeks and blood samples were drawn at weekly intervals until delivery. Sera were titrated with Ror red blood cells in a saline solution-antiglobulin method. Titers ranged from 1 to 4 by the first week after injection. In nine of 10 patients the titers were zero at 49 to 70 days after injection. With R2R2 red blood cells and LISS, papain, polybrene, or a combination of methods, Rh immune globulin could still be detected until delivery in four of the nine patients. Because these serologic methods can detect 5 ng/ml anti-D, five of 10 subjects may not have been protected for 8 to 29 days before delivery. This study indicates that a titer of ≤4 is expected for passively acquired anti-D during pregnancy, and that antepartum administration of Rh immune globulin may not always produce detectable passive antibody for as long as theoretically predicted.

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