Abstract
The disappearance kinetics of a standard dose of 300 μg of Rh immune globulin given at 28 weeks' gestation was investigated. Ten Rh-negative unsensitized women were given 300 μg of Rh immune globulin at 28 weeks and blood samples were drawn at weekly intervals until delivery. Sera were titrated with Ror red blood cells in a saline solution-antiglobulin method. Titers ranged from 1 to 4 by the first week after injection. In nine of 10 patients the titers were zero at 49 to 70 days after injection. With R2R2 red blood cells and LISS, papain, polybrene, or a combination of methods, Rh immune globulin could still be detected until delivery in four of the nine patients. Because these serologic methods can detect 5 ng/ml anti-D, five of 10 subjects may not have been protected for 8 to 29 days before delivery. This study indicates that a titer of ≤4 is expected for passively acquired anti-D during pregnancy, and that antepartum administration of Rh immune globulin may not always produce detectable passive antibody for as long as theoretically predicted.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 784-786 |
| Number of pages | 3 |
| Journal | American Journal of Obstetrics and Gynecology |
| Volume | 163 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1990 |
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Keywords
- erythroblastosis fetalis
- Rh disease
- Rh hemolytic disease
- Rh sensitization
ASJC Scopus subject areas
- Medicine(all)
- Obstetrics and Gynecology
Cite this
Postinjection kinetics of antepartum Rh immune globulin. / Witter, Frank R; Shirey, Rosetta S.; Nicol, Suzy L.; Ness, Paul Michael.
In: American Journal of Obstetrics and Gynecology, Vol. 163, No. 3, 1990, p. 784-786.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Postinjection kinetics of antepartum Rh immune globulin
AU - Witter, Frank R
AU - Shirey, Rosetta S.
AU - Nicol, Suzy L.
AU - Ness, Paul Michael
PY - 1990
Y1 - 1990
N2 - The disappearance kinetics of a standard dose of 300 μg of Rh immune globulin given at 28 weeks' gestation was investigated. Ten Rh-negative unsensitized women were given 300 μg of Rh immune globulin at 28 weeks and blood samples were drawn at weekly intervals until delivery. Sera were titrated with Ror red blood cells in a saline solution-antiglobulin method. Titers ranged from 1 to 4 by the first week after injection. In nine of 10 patients the titers were zero at 49 to 70 days after injection. With R2R2 red blood cells and LISS, papain, polybrene, or a combination of methods, Rh immune globulin could still be detected until delivery in four of the nine patients. Because these serologic methods can detect 5 ng/ml anti-D, five of 10 subjects may not have been protected for 8 to 29 days before delivery. This study indicates that a titer of ≤4 is expected for passively acquired anti-D during pregnancy, and that antepartum administration of Rh immune globulin may not always produce detectable passive antibody for as long as theoretically predicted.
AB - The disappearance kinetics of a standard dose of 300 μg of Rh immune globulin given at 28 weeks' gestation was investigated. Ten Rh-negative unsensitized women were given 300 μg of Rh immune globulin at 28 weeks and blood samples were drawn at weekly intervals until delivery. Sera were titrated with Ror red blood cells in a saline solution-antiglobulin method. Titers ranged from 1 to 4 by the first week after injection. In nine of 10 patients the titers were zero at 49 to 70 days after injection. With R2R2 red blood cells and LISS, papain, polybrene, or a combination of methods, Rh immune globulin could still be detected until delivery in four of the nine patients. Because these serologic methods can detect 5 ng/ml anti-D, five of 10 subjects may not have been protected for 8 to 29 days before delivery. This study indicates that a titer of ≤4 is expected for passively acquired anti-D during pregnancy, and that antepartum administration of Rh immune globulin may not always produce detectable passive antibody for as long as theoretically predicted.
KW - erythroblastosis fetalis
KW - Rh disease
KW - Rh hemolytic disease
KW - Rh sensitization
UR - http://www.scopus.com/inward/record.url?scp=0025187224&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025187224&partnerID=8YFLogxK
U2 - 10.1016/0002-9378(90)91068-N
DO - 10.1016/0002-9378(90)91068-N
M3 - Article
C2 - 2169705
AN - SCOPUS:0025187224
VL - 163
SP - 784
EP - 786
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
SN - 0002-9378
IS - 3
ER -