Posterior semicircular canal approach for inner ear gene delivery in neonatal mouse

Kevin Isgrig, Wade W. Chien

Research output: Contribution to journalArticle

Abstract

Inner ear gene therapy offers great promise as a potential treatment for hearing loss and dizziness. One of the critical determinants of the success of inner ear gene therapy is to find a delivery method which results in consistent transduction efficiency of targeted cell types while minimizing hearing loss. In this study, we describe the posterior semicircular canal approach as a viable method for inner ear gene delivery in neonatal mice. We show that gene delivery through the posterior semicircular canal is able to perfuse the entire inner ear. The easy anatomic identification of the posterior semicircular canal, as well as minimal manipulation of the temporal bone required, make this surgical approach an attractive option for inner ear gene delivery.

Original languageEnglish (US)
Article numbere56648
JournalJournal of Visualized Experiments
Volume2018
Issue number133
DOIs
StatePublished - Mar 2 2018

Keywords

  • Dizziness
  • Gene therapy
  • Hearing loss
  • Inner ear
  • Issue 133
  • Medicine
  • Posterior semicircular canal
  • Vestibular dysfunction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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