Population genetics and phylogenetic analysis of Colorado Borrelia burgdorferi

Douglas Norris, Barbara J B Johnson, Joseph Piesman, Gary O. Maupin, Jessica L. Clark, William C. Black IV

Research output: Contribution to journalArticle


Borrelia burgdorferi is transmitted in an enzootic cycle in Colorado between the tick Ixodes spinipalpis and the woodrat Neotoma mexicana. The genetic relationship of Colorado isolates to other B. burgdorferi isolates is unknown nor have relationships among various Colorado isolates been determined. Portions of the flagellin (fla), 66-kD protein, and outer surface protein A (ospA) genes were amplified from 71 Colorado isolates, screened for genetic variability using single strand conformation polymorphism analysis, and unique alleles were sequenced. Colorado isolates were most similar to tick isolates from California and New York isolate 25015. Genetic distances among Colorado ospA sequences were the same or higher than distances among other isolates whereas distances among fla sequences tended to be the same or lower. The index of association (I(A)) was calculated among all loci as a measure of clonality. The I(A) among Colorado isolates was similar to I(A) previously estimated among other United States isolates.

Original languageEnglish (US)
Pages (from-to)699-707
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number4
Publication statusPublished - Apr 1999
Externally publishedYes


ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Norris, D., Johnson, B. J. B., Piesman, J., Maupin, G. O., Clark, J. L., & Black IV, W. C. (1999). Population genetics and phylogenetic analysis of Colorado Borrelia burgdorferi. American Journal of Tropical Medicine and Hygiene, 60(4), 699-707.