TY - JOUR
T1 - Population genetic analysis of Enterocytozoon bieneusi in humans
AU - Li, Wei
AU - Cama, Vitaliano
AU - Feng, Yaoyu
AU - Gilman, Robert H.
AU - Bern, Caryn
AU - Zhang, Xichen
AU - Xiao, Lihua
N1 - Funding Information:
We thank Na Li and Wangeci Gate for assistance in data analysis, and National Natural Science Foundation of China for funding (No. 31110103901 and 30928019). The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
PY - 2012/3
Y1 - 2012/3
N2 - Genotyping based on sequence analysis of the ribosomal internal transcribed spacer has revealed significant genetic diversity in Enterocytozoon bieneusi. Thus far, the population genetics of E. bieneusi and its significance in the epidemiology of microsporidiosis have not been examined. In this study, a multilocus sequence typing of E. bieneusi in AIDS patients in Lima, Peru was conducted, using 72 specimens previously genotyped as A, D, IV, EbpC, WL11, Peru7, Peru8, Peru10 and Peru11 at the internal transcribed spacer locus. Altogether, 39 multilocus genotypes were identified among the 72 specimens. The observation of strong intragenic linkage disequilibria and limited genetic recombination among markers were indicative of an overall clonal population structure of E. bieneusi. Measures of pair-wise intergenic linkage disequilibria and a standardised index of association (IAS) based on allelic profile data further supported this conclusion. Both sequence-based and allelic profile-based phylogenetic analyses showed the presence of two genetically isolated groups in the study population, one (group 1) containing isolates of the anthroponotic internal transcribed spacer genotype A, and the other (group 2) containing isolates of multiple internal transcribed spacer genotypes (mainly genotypes D and IV) with zoonotic potential. The measurement of linkage disequilibria and recombination indicated group 2 had a clonal population structure, whereas group 1 had an epidemic population structure. The formation of the two sub-populations was confirmed by STRUCTURE and Wright's fixation index (F ST) analyses. The data highlight the power of MLST in understanding the epidemiology of E. bieneusi.
AB - Genotyping based on sequence analysis of the ribosomal internal transcribed spacer has revealed significant genetic diversity in Enterocytozoon bieneusi. Thus far, the population genetics of E. bieneusi and its significance in the epidemiology of microsporidiosis have not been examined. In this study, a multilocus sequence typing of E. bieneusi in AIDS patients in Lima, Peru was conducted, using 72 specimens previously genotyped as A, D, IV, EbpC, WL11, Peru7, Peru8, Peru10 and Peru11 at the internal transcribed spacer locus. Altogether, 39 multilocus genotypes were identified among the 72 specimens. The observation of strong intragenic linkage disequilibria and limited genetic recombination among markers were indicative of an overall clonal population structure of E. bieneusi. Measures of pair-wise intergenic linkage disequilibria and a standardised index of association (IAS) based on allelic profile data further supported this conclusion. Both sequence-based and allelic profile-based phylogenetic analyses showed the presence of two genetically isolated groups in the study population, one (group 1) containing isolates of the anthroponotic internal transcribed spacer genotype A, and the other (group 2) containing isolates of multiple internal transcribed spacer genotypes (mainly genotypes D and IV) with zoonotic potential. The measurement of linkage disequilibria and recombination indicated group 2 had a clonal population structure, whereas group 1 had an epidemic population structure. The formation of the two sub-populations was confirmed by STRUCTURE and Wright's fixation index (F ST) analyses. The data highlight the power of MLST in understanding the epidemiology of E. bieneusi.
KW - Enterocytozoon bieneusi
KW - Gene diversity
KW - Linkage disequilibrium
KW - Multilocus sequence typing
KW - Population genetics
KW - Population structure
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U2 - 10.1016/j.ijpara.2012.01.003
DO - 10.1016/j.ijpara.2012.01.003
M3 - Article
C2 - 22534008
AN - SCOPUS:84862800281
SN - 0020-7519
VL - 42
SP - 287
EP - 293
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 3
ER -