Population genetic analysis of Enterocytozoon bieneusi in humans

Wei Li, Vitaliano Cama, Yaoyu Feng, Robert H Gilman, Caryn Bern, Xichen Zhang, Lihua Xiao

Research output: Contribution to journalArticle

Abstract

Genotyping based on sequence analysis of the ribosomal internal transcribed spacer has revealed significant genetic diversity in Enterocytozoon bieneusi. Thus far, the population genetics of E. bieneusi and its significance in the epidemiology of microsporidiosis have not been examined. In this study, a multilocus sequence typing of E. bieneusi in AIDS patients in Lima, Peru was conducted, using 72 specimens previously genotyped as A, D, IV, EbpC, WL11, Peru7, Peru8, Peru10 and Peru11 at the internal transcribed spacer locus. Altogether, 39 multilocus genotypes were identified among the 72 specimens. The observation of strong intragenic linkage disequilibria and limited genetic recombination among markers were indicative of an overall clonal population structure of E. bieneusi. Measures of pair-wise intergenic linkage disequilibria and a standardised index of association (IAS) based on allelic profile data further supported this conclusion. Both sequence-based and allelic profile-based phylogenetic analyses showed the presence of two genetically isolated groups in the study population, one (group 1) containing isolates of the anthroponotic internal transcribed spacer genotype A, and the other (group 2) containing isolates of multiple internal transcribed spacer genotypes (mainly genotypes D and IV) with zoonotic potential. The measurement of linkage disequilibria and recombination indicated group 2 had a clonal population structure, whereas group 1 had an epidemic population structure. The formation of the two sub-populations was confirmed by STRUCTURE and Wright's fixation index (F ST) analyses. The data highlight the power of MLST in understanding the epidemiology of E. bieneusi.

Original languageEnglish (US)
Pages (from-to)287-293
Number of pages7
JournalInternational Journal for Parasitology
Volume42
Issue number3
DOIs
StatePublished - Mar 2012

Fingerprint

Enterocytozoon
Population Genetics
Linkage Disequilibrium
Genotype
Genetic Recombination
Population
Epidemiology
Microsporidiosis
Multilocus Sequence Typing
Peru
Zoonoses
Population Groups
Sequence Analysis
Acquired Immunodeficiency Syndrome
Observation

Keywords

  • Enterocytozoon bieneusi
  • Gene diversity
  • Linkage disequilibrium
  • Multilocus sequence typing
  • Population genetics
  • Population structure

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Population genetic analysis of Enterocytozoon bieneusi in humans. / Li, Wei; Cama, Vitaliano; Feng, Yaoyu; Gilman, Robert H; Bern, Caryn; Zhang, Xichen; Xiao, Lihua.

In: International Journal for Parasitology, Vol. 42, No. 3, 03.2012, p. 287-293.

Research output: Contribution to journalArticle

Li, Wei ; Cama, Vitaliano ; Feng, Yaoyu ; Gilman, Robert H ; Bern, Caryn ; Zhang, Xichen ; Xiao, Lihua. / Population genetic analysis of Enterocytozoon bieneusi in humans. In: International Journal for Parasitology. 2012 ; Vol. 42, No. 3. pp. 287-293.
@article{20498e529dcb4542bd2795cce529ef77,
title = "Population genetic analysis of Enterocytozoon bieneusi in humans",
abstract = "Genotyping based on sequence analysis of the ribosomal internal transcribed spacer has revealed significant genetic diversity in Enterocytozoon bieneusi. Thus far, the population genetics of E. bieneusi and its significance in the epidemiology of microsporidiosis have not been examined. In this study, a multilocus sequence typing of E. bieneusi in AIDS patients in Lima, Peru was conducted, using 72 specimens previously genotyped as A, D, IV, EbpC, WL11, Peru7, Peru8, Peru10 and Peru11 at the internal transcribed spacer locus. Altogether, 39 multilocus genotypes were identified among the 72 specimens. The observation of strong intragenic linkage disequilibria and limited genetic recombination among markers were indicative of an overall clonal population structure of E. bieneusi. Measures of pair-wise intergenic linkage disequilibria and a standardised index of association (IAS) based on allelic profile data further supported this conclusion. Both sequence-based and allelic profile-based phylogenetic analyses showed the presence of two genetically isolated groups in the study population, one (group 1) containing isolates of the anthroponotic internal transcribed spacer genotype A, and the other (group 2) containing isolates of multiple internal transcribed spacer genotypes (mainly genotypes D and IV) with zoonotic potential. The measurement of linkage disequilibria and recombination indicated group 2 had a clonal population structure, whereas group 1 had an epidemic population structure. The formation of the two sub-populations was confirmed by STRUCTURE and Wright's fixation index (F ST) analyses. The data highlight the power of MLST in understanding the epidemiology of E. bieneusi.",
keywords = "Enterocytozoon bieneusi, Gene diversity, Linkage disequilibrium, Multilocus sequence typing, Population genetics, Population structure",
author = "Wei Li and Vitaliano Cama and Yaoyu Feng and Gilman, {Robert H} and Caryn Bern and Xichen Zhang and Lihua Xiao",
year = "2012",
month = "3",
doi = "10.1016/j.ijpara.2012.01.003",
language = "English (US)",
volume = "42",
pages = "287--293",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Population genetic analysis of Enterocytozoon bieneusi in humans

AU - Li, Wei

AU - Cama, Vitaliano

AU - Feng, Yaoyu

AU - Gilman, Robert H

AU - Bern, Caryn

AU - Zhang, Xichen

AU - Xiao, Lihua

PY - 2012/3

Y1 - 2012/3

N2 - Genotyping based on sequence analysis of the ribosomal internal transcribed spacer has revealed significant genetic diversity in Enterocytozoon bieneusi. Thus far, the population genetics of E. bieneusi and its significance in the epidemiology of microsporidiosis have not been examined. In this study, a multilocus sequence typing of E. bieneusi in AIDS patients in Lima, Peru was conducted, using 72 specimens previously genotyped as A, D, IV, EbpC, WL11, Peru7, Peru8, Peru10 and Peru11 at the internal transcribed spacer locus. Altogether, 39 multilocus genotypes were identified among the 72 specimens. The observation of strong intragenic linkage disequilibria and limited genetic recombination among markers were indicative of an overall clonal population structure of E. bieneusi. Measures of pair-wise intergenic linkage disequilibria and a standardised index of association (IAS) based on allelic profile data further supported this conclusion. Both sequence-based and allelic profile-based phylogenetic analyses showed the presence of two genetically isolated groups in the study population, one (group 1) containing isolates of the anthroponotic internal transcribed spacer genotype A, and the other (group 2) containing isolates of multiple internal transcribed spacer genotypes (mainly genotypes D and IV) with zoonotic potential. The measurement of linkage disequilibria and recombination indicated group 2 had a clonal population structure, whereas group 1 had an epidemic population structure. The formation of the two sub-populations was confirmed by STRUCTURE and Wright's fixation index (F ST) analyses. The data highlight the power of MLST in understanding the epidemiology of E. bieneusi.

AB - Genotyping based on sequence analysis of the ribosomal internal transcribed spacer has revealed significant genetic diversity in Enterocytozoon bieneusi. Thus far, the population genetics of E. bieneusi and its significance in the epidemiology of microsporidiosis have not been examined. In this study, a multilocus sequence typing of E. bieneusi in AIDS patients in Lima, Peru was conducted, using 72 specimens previously genotyped as A, D, IV, EbpC, WL11, Peru7, Peru8, Peru10 and Peru11 at the internal transcribed spacer locus. Altogether, 39 multilocus genotypes were identified among the 72 specimens. The observation of strong intragenic linkage disequilibria and limited genetic recombination among markers were indicative of an overall clonal population structure of E. bieneusi. Measures of pair-wise intergenic linkage disequilibria and a standardised index of association (IAS) based on allelic profile data further supported this conclusion. Both sequence-based and allelic profile-based phylogenetic analyses showed the presence of two genetically isolated groups in the study population, one (group 1) containing isolates of the anthroponotic internal transcribed spacer genotype A, and the other (group 2) containing isolates of multiple internal transcribed spacer genotypes (mainly genotypes D and IV) with zoonotic potential. The measurement of linkage disequilibria and recombination indicated group 2 had a clonal population structure, whereas group 1 had an epidemic population structure. The formation of the two sub-populations was confirmed by STRUCTURE and Wright's fixation index (F ST) analyses. The data highlight the power of MLST in understanding the epidemiology of E. bieneusi.

KW - Enterocytozoon bieneusi

KW - Gene diversity

KW - Linkage disequilibrium

KW - Multilocus sequence typing

KW - Population genetics

KW - Population structure

UR - http://www.scopus.com/inward/record.url?scp=84862800281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862800281&partnerID=8YFLogxK

U2 - 10.1016/j.ijpara.2012.01.003

DO - 10.1016/j.ijpara.2012.01.003

M3 - Article

C2 - 22534008

AN - SCOPUS:84862800281

VL - 42

SP - 287

EP - 293

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 3

ER -