Polystyrene microbridges used in sitting-drop crystallization release 1,4-diphenyl-2-butene, a novel inhibitor of human MAO B

Frantisek Hubálek, Claudia Binda, Min Li, Andrea Mattevi, Dale E. Edmondson

Research output: Contribution to journalArticle

Abstract

In the course of protein-structure determinations of the membrane-bound enzyme monoamine oxidase B (MAO B) by X-ray crystallography, a compound was found in the active site of the enzyme that consists of two phenyl rings separated by four C atoms. This compound was identified by chromatography and by mass spectrometry to be 1,4-diphenyl-2-butene and found to be a component of the polystyrene microbridges that are used in protein crystallization. This compound is present at a level of ∼0.3 mg (∼1.5 μmol) per microbridge and functions as a competitive inhibitor of MAO B with a K i of 35 μM. The presence of detergents in the crystallization solutions facilitates the extraction of this compound from the polymer medium.

Original languageEnglish (US)
Pages (from-to)1874-1876
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume59
Issue number10
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

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oxidase
Polystyrenes
Monoamine Oxidase
butenes
Crystallization
inhibitors
enzymes
polystyrene
crystallization
proteins
detergents
X ray crystallography
X Ray Crystallography
Enzymes
chromatography
Chromatography
Detergents
crystallography
Mass spectrometry
Mass Spectrometry

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Polystyrene microbridges used in sitting-drop crystallization release 1,4-diphenyl-2-butene, a novel inhibitor of human MAO B. / Hubálek, Frantisek; Binda, Claudia; Li, Min; Mattevi, Andrea; Edmondson, Dale E.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 59, No. 10, 01.10.2003, p. 1874-1876.

Research output: Contribution to journalArticle

Hubálek, Frantisek ; Binda, Claudia ; Li, Min ; Mattevi, Andrea ; Edmondson, Dale E. / Polystyrene microbridges used in sitting-drop crystallization release 1,4-diphenyl-2-butene, a novel inhibitor of human MAO B. In: Acta Crystallographica Section D: Biological Crystallography. 2003 ; Vol. 59, No. 10. pp. 1874-1876.
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