Polysome analysis of mammalian cells

Shan L. He, Rachel Green

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

To assess the global translational level of mammalian cells (see similar protocols for bacteria and yeast on Analysis of polysomes from bacteria, Polysome Profile Analysis - Yeast and Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc.
Pages183-192
Number of pages10
ISBN (Print)9780124200371
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume530
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cell lysate
  • Genome-wide analysis techniques
  • Immobilize ribosomes
  • Mammalian cells
  • Polysome analysis Size fractionation
  • Sucrose density gradient centrifugation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Polysome analysis of mammalian cells'. Together they form a unique fingerprint.

  • Cite this

    He, S. L., & Green, R. (2013). Polysome analysis of mammalian cells. In Laboratory Methods in Enzymology: RNA (pp. 183-192). (Methods in Enzymology; Vol. 530). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420037-1.00010-5