Polysome analysis of mammalian cells

Shan L. He, Rachel Green

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations


To assess the global translational level of mammalian cells (see similar protocols for bacteria and yeast on Analysis of polysomes from bacteria, Polysome Profile Analysis - Yeast and Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc.
Number of pages10
ISBN (Print)9780124200371
StatePublished - 2013

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Cell lysate
  • Genome-wide analysis techniques
  • Immobilize ribosomes
  • Mammalian cells
  • Polysome analysis Size fractionation
  • Sucrose density gradient centrifugation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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