Polyreactive monoclonal autoantibodies in multiple sclerosis: Functional selection from phage display library and characterization by deep sequencing analysis

Y. A. Lomakin, M. Yu Zakharova, A. A. Belogurov, N. A. Bykova, M. A. Dronina, A. E. Tupikin, V. D. Knorre, A. N. Boyko, Alexander Favorov, M. R. Kabilov, N. A. Ponomarenko, A. G. Gabibov

Research output: Contribution to journalArticle

Abstract

Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system that primarily affects young and middle-aged people. It is widely accepted that B lymphocyte activation is required for MS progression. Despite the fact that the exact triggering mechanisms of MS remain enigmatic, one may suggest that MS can be induced by viral or bacterial infection in combination with specific genetic and environmental factors. Using deep sequencing and functional selection methodologies we characterized clones of poly- and cross-reactive antibodies that are capable of simultaneous recognition of viral proteins and autoantigens. The latter, in turn, possibly may trigger MS progression through molecular mimicry. It was identified that two cross-reactive antigens are probably recognized by light or heavy chains individually. According to the high structural homology between selected autoantibodies and a number of various antiviral IgGs, we suggest that a wide range of pathogens, instead of a single virus, be regarded as possible triggers of MS.

Original languageEnglish (US)
Pages (from-to)94-104
Number of pages11
JournalActa Naturae
Volume5
Issue number19
StatePublished - 2013

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High-Throughput Nucleotide Sequencing
Bacteriophages
Lymphocytes
Autoantigens
Neurology
Viral Proteins
Pathogens
Viruses
Autoantibodies
Libraries
Multiple Sclerosis
Antiviral Agents
Chemical activation
Display devices
Antigens
Antibodies
Molecular Mimicry
Demyelinating Diseases
Virus Diseases
Lymphocyte Activation

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Molecular Medicine

Cite this

Lomakin, Y. A., Zakharova, M. Y., Belogurov, A. A., Bykova, N. A., Dronina, M. A., Tupikin, A. E., ... Gabibov, A. G. (2013). Polyreactive monoclonal autoantibodies in multiple sclerosis: Functional selection from phage display library and characterization by deep sequencing analysis. Acta Naturae, 5(19), 94-104.

Polyreactive monoclonal autoantibodies in multiple sclerosis : Functional selection from phage display library and characterization by deep sequencing analysis. / Lomakin, Y. A.; Zakharova, M. Yu; Belogurov, A. A.; Bykova, N. A.; Dronina, M. A.; Tupikin, A. E.; Knorre, V. D.; Boyko, A. N.; Favorov, Alexander; Kabilov, M. R.; Ponomarenko, N. A.; Gabibov, A. G.

In: Acta Naturae, Vol. 5, No. 19, 2013, p. 94-104.

Research output: Contribution to journalArticle

Lomakin, YA, Zakharova, MY, Belogurov, AA, Bykova, NA, Dronina, MA, Tupikin, AE, Knorre, VD, Boyko, AN, Favorov, A, Kabilov, MR, Ponomarenko, NA & Gabibov, AG 2013, 'Polyreactive monoclonal autoantibodies in multiple sclerosis: Functional selection from phage display library and characterization by deep sequencing analysis', Acta Naturae, vol. 5, no. 19, pp. 94-104.
Lomakin, Y. A. ; Zakharova, M. Yu ; Belogurov, A. A. ; Bykova, N. A. ; Dronina, M. A. ; Tupikin, A. E. ; Knorre, V. D. ; Boyko, A. N. ; Favorov, Alexander ; Kabilov, M. R. ; Ponomarenko, N. A. ; Gabibov, A. G. / Polyreactive monoclonal autoantibodies in multiple sclerosis : Functional selection from phage display library and characterization by deep sequencing analysis. In: Acta Naturae. 2013 ; Vol. 5, No. 19. pp. 94-104.
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