Polyproline II structure in a sequence of seven alanine residues

Zhengshuang Shi, C. Anders Olson, George D Rose, Robert L. Baldwin, Neville R. Kallenbach

Research output: Contribution to journalArticle

Abstract

A sequence of seven alanine residues - too short to form an a-helix and whose side chains do not interact with each other - is a particularly simple model for testing the common description of denatured proteins as structureless random coils. The 3JHNα coupling constants of individual alanine residues have been measured from 2 to 56°C by using isotopically labeled samples. The results display a thermal transition between different backbone conformations, which is confirmed by CD spectra. The NMR results suggest that polyproline II 11 is the dominant conformation at 2°C and the content β strand is increased by approximately 10% at 55°C relative to that at 2°C. The polyproline II conformation is consistent with recent studies of short alanine peptides, including structure prediction by ab initio quantum mechanics and solution structures for both a blocked alanine dipeptide and an alanine tripeptide. CD and other optical spectroscopies have found structure in longer "random coil" peptides and have implicated polyproline II, which is a major backbone conformation in residues within loop regions of protein structures. Our result suggests that the backbone conformational entropy in alanine peptides is considerably smaller than estimated by the random coil model. New thermodynamic data confirm this suggestion: the entropy loss on alanine helix formation is only 2.2 entropy units per residue.

Original languageEnglish (US)
Pages (from-to)9190-9195
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number14
DOIs
StatePublished - Jul 9 2002

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Alanine
Entropy
Peptides
Dipeptides
polyproline
Mechanics
Thermodynamics
Spectrum Analysis
Proteins
Hot Temperature

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Polyproline II structure in a sequence of seven alanine residues. / Shi, Zhengshuang; Anders Olson, C.; Rose, George D; Baldwin, Robert L.; Kallenbach, Neville R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 14, 09.07.2002, p. 9190-9195.

Research output: Contribution to journalArticle

Shi, Zhengshuang ; Anders Olson, C. ; Rose, George D ; Baldwin, Robert L. ; Kallenbach, Neville R. / Polyproline II structure in a sequence of seven alanine residues. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 14. pp. 9190-9195.
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