The traditionally derived syrup of Opuntia ficus-indica fruit is commonly used in homemade confectionery. Herein, the aqueous-acetone extract prepared from the Tunisian O. ficus-indica syrup was investigated. The qualitatively and quantitatively polyphenolic content was analysed using reversed-phase high-performance liquid chromatography-diode array detection (RP-HPLC-DAD) coupled to electrospray ionisation-mass spectrometry (ESI-MS). The extract contained 19. 95 ± 2. 01 mg phenolics per gram of fresh starting material with isorhamnetin 3-O-robinobioside as the major compound (22. 76%). The syrup extract showed strong antioxidant potentials as assessed by both ABTS and DPPH functional methods. It exhibited effective antimicrobial activity, particularly against Staphylococcus aureus and Staphylococcus epidermidis with a minimal bactericide concentration (MBC) of 1. 3 mg phenolics/ml. Furthermore, at final concentrations in the range of 41. 38-186. 25 μg polyphenols/ml, the extract decreased human SH-SY5Y neuroblastoma and 3T3 fibroblast in vitro cell viability in a dose- and time-dependent manner compared to non-treated control cells. The observed effects were significantly (P < 0. 05) high against cancer lines. Extract concentrations higher than 106. 43 μg/ml reduced cancer cells viability to 50-60% 1-3 h post-treatment. Further in vivo insight studies should emphasise and validate the herein obtained results.
- Cancer cells
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science
- Applied Microbiology and Biotechnology
- Safety, Risk, Reliability and Quality
- Safety Research