Polyphenolic Extract of Barbary-Fig (Opuntia ficus-indica) Syrup: RP-HPLC-ESI-MS Analysis and Determination of Antioxidant, Antimicrobial and Cancer-Cells Cytotoxic Potentials

Karima Dhaouadi, Faten Raboudi, Lorena Funez-Gomez, David Pamies, Carmen Estevan, Mohamed Hamdaoui, Sami Fattouch

Research output: Contribution to journalArticle

Abstract

The traditionally derived syrup of Opuntia ficus-indica fruit is commonly used in homemade confectionery. Herein, the aqueous-acetone extract prepared from the Tunisian O. ficus-indica syrup was investigated. The qualitatively and quantitatively polyphenolic content was analysed using reversed-phase high-performance liquid chromatography-diode array detection (RP-HPLC-DAD) coupled to electrospray ionisation-mass spectrometry (ESI-MS). The extract contained 19. 95 ± 2. 01 mg phenolics per gram of fresh starting material with isorhamnetin 3-O-robinobioside as the major compound (22. 76%). The syrup extract showed strong antioxidant potentials as assessed by both ABTS and DPPH functional methods. It exhibited effective antimicrobial activity, particularly against Staphylococcus aureus and Staphylococcus epidermidis with a minimal bactericide concentration (MBC) of 1. 3 mg phenolics/ml. Furthermore, at final concentrations in the range of 41. 38-186. 25 μg polyphenols/ml, the extract decreased human SH-SY5Y neuroblastoma and 3T3 fibroblast in vitro cell viability in a dose- and time-dependent manner compared to non-treated control cells. The observed effects were significantly (P < 0. 05) high against cancer lines. Extract concentrations higher than 106. 43 μg/ml reduced cancer cells viability to 50-60% 1-3 h post-treatment. Further in vivo insight studies should emphasise and validate the herein obtained results.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalFood Analytical Methods
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2013

Keywords

  • Antimicrobial
  • Antioxidant
  • Cancer cells
  • LC-ESI-MS
  • Polyphenols
  • Syrup

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

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