Polypeptides of cells transformed by RNA or DNA tumor viruses

M. Strand, J. T. August

Research output: Contribution to journalArticle

Abstract

Proteins solubilized from normal BALB/3T3 cells and BALB/3T3 transformed by simian virus 40 or Kirsten sarcoma virus have been analyzed by two-dimensional gel electrophoresis and tryptic peptide mapping. A large fraction of polypeptides of the virus-transformed cells, about 30%, were different from normal cells. In contrast to the marked differences between normal and transformed cells, the polypeptides of the DNA and RNA virus-transformed cells were almost identical. These findings were observed with polypeptides stained by Coomassie Blue, or labeled with [14C]glucosamine or [35S]methionine. Pulse chase analysis showed that most of the polypeptides were stable during 20 hr of incubation. The identity of several polypeptides was confirmed by tryptic peptide mapping.

Original languageEnglish (US)
Pages (from-to)2729-2733
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number7
DOIs
StatePublished - 1977

ASJC Scopus subject areas

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