TY - JOUR
T1 - Polymorphisms detected by random PCR distinguish between different chromosomal forms of Anopheles gambiae
AU - Favia, Guido
AU - Dimopoulos, George
AU - Della Torre, Alessandra
AU - Touré, Yeya T.
AU - Coluzzi, Mario
AU - Louis, Christos
PY - 1994/10/25
Y1 - 1994/10/25
N2 - We have applied PCR amplification using random primers to distinguish between incipient species of the malaria vector Anopheles gambiae. Individuals belonging to three chromosomally characterized West African forms of this mosquito, which are important epidemiologically as they differ in vectorial capacity, were sampled both from laboratory stocks and from wild populations collected in three localities. The techniques used allowed for the unambiguous classification of the mosquitoes, providing a tool for rapid and efficient diagnosis, which previously relied on cytological examination of polytene chromosomes.
AB - We have applied PCR amplification using random primers to distinguish between incipient species of the malaria vector Anopheles gambiae. Individuals belonging to three chromosomally characterized West African forms of this mosquito, which are important epidemiologically as they differ in vectorial capacity, were sampled both from laboratory stocks and from wild populations collected in three localities. The techniques used allowed for the unambiguous classification of the mosquitoes, providing a tool for rapid and efficient diagnosis, which previously relied on cytological examination of polytene chromosomes.
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U2 - 10.1073/pnas.91.22.10315
DO - 10.1073/pnas.91.22.10315
M3 - Article
C2 - 7937947
AN - SCOPUS:0028143594
SN - 0027-8424
VL - 91
SP - 10315
EP - 10319
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -