Polymorphisms detected by random PCR distinguish between different chromosomal forms of Anopheles gambiae

Guido Favia, George Dimopoulos, Alessandra Della Torre, Yeya T. Touré, Mario Coluzzi, Christos Louis

Research output: Contribution to journalArticle

Abstract

We have applied PCR amplification using random primers to distinguish between incipient species of the malaria vector Anopheles gambiae. Individuals belonging to three chromosomally characterized West African forms of this mosquito, which are important epidemiologically as they differ in vectorial capacity, were sampled both from laboratory stocks and from wild populations collected in three localities. The techniques used allowed for the unambiguous classification of the mosquitoes, providing a tool for rapid and efficient diagnosis, which previously relied on cytological examination of polytene chromosomes.

Original languageEnglish (US)
Pages (from-to)10315-10319
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number22
DOIs
StatePublished - Oct 25 1994
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Polymorphisms detected by random PCR distinguish between different chromosomal forms of Anopheles gambiae'. Together they form a unique fingerprint.

  • Cite this