Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients

R. Mauritz, I. J. Beumer, S. Marsh, H. L. McLeod, K. Smid, C. J. Van Groeningen, H. M. Pinedo, G. J. Peters

Research output: Contribution to journal › Article

Abstract

In a panel of 18 colon cancer cell lines we found that the thymidylate synthase (TS) genotype was related to TS enzyme activity, but not to TS protein and mRNA levels. In addition, no relation with drug sensitivity was observed. TS genotyping of different tissues from 78 colorectal cancer patients revealed a high level of homology in polymorphic status between normal and malignant tissues and the heterozygous genotype to be the most frequent.

Original languageEnglish (US)
Pages (from-to)1381-1384
Number of pages4
JournalNucleosides, Nucleotides and Nucleic Acids
Volume23
Issue number8-9
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Thymidylate Synthase
Polymorphism
Colonic Neoplasms
Colorectal Neoplasms
Genes
Cells
Tissue
Cell Line
Genotype
Enzyme activity
Messenger RNA
Enzymes
Pharmaceutical Preparations
Proteins

Keywords

  • 5-Fluorouracil
  • Colorectal carcinoma
  • Polymorphism
  • Thymidylate synthase

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients. / Mauritz, R.; Beumer, I. J.; Marsh, S.; McLeod, H. L.; Smid, K.; Van Groeningen, C. J.; Pinedo, H. M.; Peters, G. J.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 23, No. 8-9, 2004, p. 1381-1384.

Research output: Contribution to journal › Article

Mauritz, R, Beumer, IJ, Marsh, S, McLeod, HL, Smid, K, Van Groeningen, CJ, Pinedo, HM & Peters, GJ 2004, 'Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients', Nucleosides, Nucleotides and Nucleic Acids, vol. 23, no. 8-9, pp. 1381-1384. https://doi.org/10.1081/NCN-200027634
Mauritz R, Beumer IJ, Marsh S, McLeod HL, Smid K, Van Groeningen CJ et al. Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients. Nucleosides, Nucleotides and Nucleic Acids. 2004;23(8-9):1381-1384. https://doi.org/10.1081/NCN-200027634
Mauritz, R. ; Beumer, I. J. ; Marsh, S. ; McLeod, H. L. ; Smid, K. ; Van Groeningen, C. J. ; Pinedo, H. M. ; Peters, G. J. / Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients. In: Nucleosides, Nucleotides and Nucleic Acids. 2004 ; Vol. 23, No. 8-9. pp. 1381-1384.
@article{c0b41ec99c0b48ebb32316c28aea3c26,
title = "Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients",
abstract = "In a panel of 18 colon cancer cell lines we found that the thymidylate synthase (TS) genotype was related to TS enzyme activity, but not to TS protein and mRNA levels. In addition, no relation with drug sensitivity was observed. TS genotyping of different tissues from 78 colorectal cancer patients revealed a high level of homology in polymorphic status between normal and malignant tissues and the heterozygous genotype to be the most frequent.",
keywords = "5-Fluorouracil, Colorectal carcinoma, Polymorphism, Thymidylate synthase",
author = "R. Mauritz and Beumer, {I. J.} and S. Marsh and McLeod, {H. L.} and K. Smid and {Van Groeningen}, {C. J.} and Pinedo, {H. M.} and Peters, {G. J.}",
year = "2004",
doi = "10.1081/NCN-200027634",
language = "English (US)",
volume = "23",
pages = "1381--1384",
journal = "Nucleosides and Nucleotides",
issn = "0732-8311",
publisher = "Taylor and Francis Ltd.",
number = "8-9",

}

TY - JOUR

T1 - Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients

AU - Mauritz, R.

AU - Beumer, I. J.

AU - Marsh, S.

AU - McLeod, H. L.

AU - Smid, K.

AU - Van Groeningen, C. J.

AU - Pinedo, H. M.

AU - Peters, G. J.

PY - 2004

Y1 - 2004

N2 - In a panel of 18 colon cancer cell lines we found that the thymidylate synthase (TS) genotype was related to TS enzyme activity, but not to TS protein and mRNA levels. In addition, no relation with drug sensitivity was observed. TS genotyping of different tissues from 78 colorectal cancer patients revealed a high level of homology in polymorphic status between normal and malignant tissues and the heterozygous genotype to be the most frequent.

AB - In a panel of 18 colon cancer cell lines we found that the thymidylate synthase (TS) genotype was related to TS enzyme activity, but not to TS protein and mRNA levels. In addition, no relation with drug sensitivity was observed. TS genotyping of different tissues from 78 colorectal cancer patients revealed a high level of homology in polymorphic status between normal and malignant tissues and the heterozygous genotype to be the most frequent.

KW - 5-Fluorouracil

KW - Colorectal carcinoma

KW - Polymorphism

KW - Thymidylate synthase

UR - http://www.scopus.com/inward/record.url?scp=10344250989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10344250989&partnerID=8YFLogxK

U2 - 10.1081/NCN-200027634

DO - 10.1081/NCN-200027634

M3 - Article

C2 - 15571263

AN - SCOPUS:10344250989

VL - 23

SP - 1381

EP - 1384

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

SN - 0732-8311

IS - 8-9

ER -

Access to Document