Polymerization of actin does not regulate desensitization in human basophils

Donald Macglashan, Natalia Vilariño

Research output: Contribution to journalArticle

Abstract

Previous studies have suggested that maintenance of IgE-mediated signaling results from regulation of the activity of signaling complexes by actin polymerization. This process is also hypothesized to be related to desensitization of basophils and mast cells. Recent studies demonstrated that any signaling process dependent on syk or PI-3K activity cannot be a mechanism of desensitization, and in this context, syk and PI-3K inhibitors were found to inhibit actin polymerization. Inhibitors of actin polymerization were tested for their effect on desensitization of human peripheral blood basophils. Latrunculin A, in particular, removed all resting and stimulated f-actin but did not inhibit desensitization. Cytochalasin D and latrunculin A also did not reverse the loss of syk phosphorylation that accompanies desensitization. These results demonstrate that desensitization mechanisms are not dependent on actin polymerization. In this context, it was also shown that progressive immobilization of FcεRI during aggregation was sensitive to syk or actin polymerization inhibition. Therefore, desensitization is also not dependent on receptor immobilization. These studies demonstrate that desensitization is not the result of two signaling pathways once considered relevant to down-regulation of IgE-mediated signaling.

Original languageEnglish (US)
Pages (from-to)627-637
Number of pages11
JournalJournal of Leukocyte Biology
Volume85
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Basophils
Polymerization
Actins
Phosphatidylinositol 3-Kinases
Immobilization
Immunoglobulin E
Cytochalasin D
Mast Cells
Down-Regulation
Maintenance
Phosphorylation

Keywords

  • FcεRI
  • Histamine release
  • Signal termination

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Polymerization of actin does not regulate desensitization in human basophils. / Macglashan, Donald; Vilariño, Natalia.

In: Journal of Leukocyte Biology, Vol. 85, No. 4, 01.04.2009, p. 627-637.

Research output: Contribution to journalArticle

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