Polymeric nanofiber coating with tunable combinatorial antibiotic delivery prevents biofilm-associated infection in vivo

Alyssa G. Ashbaugh, Xuesong Jiang, Jesse Zheng, Andrew S. Tsai, Woo Shin Kim, John M. Thompson, Robert J. Miller, Jonathan H. Shahbazian, Yu Wang, Carly A. Dillen, Alvaro Ordonez Suarez, Yong S. Chang, Sanjay Jain, Lynne C Jones, Robert Steven Sterling, Hai Quan Mao, Lloyd S Miller

Research output: Contribution to journalArticle

Abstract

Bacterial biofilm formation is a major complication of implantable medical devices that results in therapeutically challenging chronic infections, especially in cases involving antibiotic-resistant bacteria. As an approach to prevent these infections, an electrospun composite coating comprised of poly(lactic-coglycolic acid) (PLGA) nanofibers embedded in a poly(e-caprolactone) (PCL) film was developed to locally codeliver combinatorial antibiotics from the implant surface. The release of each antibiotic could be adjusted by loading each drug into the different polymers or by varying PLGA:PCL polymer ratios. In a mouse model of biofilm-associated orthopedic-implant infection, three different combinations of antibiotic-loaded coatings were highly effective in preventing infection of the bone/joint tissue and implant biofilm formation and were biocompatible with enhanced osseointegration. This nanofiber composite-coating technology could be used to tailor the delivery of combinatorial antimicrobial agents from various metallic implantable devices or prostheses to effectively decrease biofilm-associated infections in patients.

Original languageEnglish (US)
Pages (from-to)E6919-E6928
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number45
DOIs
Publication statusPublished - Nov 8 2016

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Keywords

  • Antibiotic
  • Biofilm
  • Delivery
  • Infection
  • Nanofiber

ASJC Scopus subject areas

  • General

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